Correlation of complement fragment C5a with inflammatory cytokines in the vitreous of patients with proliferative diabetic retinopathy

详细信息    查看全文

• 作者：Daisuke Muramatsu (1)
  Yoshihiro Wakabayashi (1)
  Yoshihiko Usui (1)
  Yoko Okunuki (1)
  Takeshi Kezuka (1)
  Hiroshi Goto (1)
  
• 关键词：Complement factor ; Proliferative diabetic retinopathy ; C5a ; Vascular endothelial growth factor ; Monocyte chemotactic protein ; 1
• 刊名：Graefe's Archive for Clinical and Experimental Ophthalmology
• 出版年：2013
• 出版时间：January 2013
• 年：2013
• 卷：251
• 期：1
• 页码：15-17
• 全文大小：135KB
• 参考文献：1. Accardo-Palumbo A, Triolo G, Colonna-Romano G, Potestio M, Carbone M, Ferrante A, Giardina E, Caimi G, Triolo G (2000) Glucose-induced loss of glycosyl-phosphatidylinositol-anchored membrane regulators of complement activation (CD59, CD55) by in vitro cultured human umbilical vein endothelial cells. Diabetologia 43:1039-047 CrossRef
  2. Zhang J, Gerhardinger C, Lorenzi M (2002) Early complement activation and decreased levels of glycosylphosphatidylinositol-anchored complement inhibitors in human and experimental diabetic retinopathy. Diabetes 51:3499-504 CrossRef
  3. Dagher Z, Park YS, Asnaghi V, Hoehn T, Gerhardinger C, Lorenzi M (2004) Studies of rat and human retinas predict a role for the polyol pathway in human diabetic retinopathy. Diabetes 53:2404-411 CrossRef
  4. K?hl J (2001) Anaphylatoxins and infectious and non-infectious inflammatory diseases. Mol Immunol 38:175-87 CrossRef
  5. Cortright DN, Meade R, Waters SM, Chenard BL, Krause JE (2009) C5a, but not C3a, increases VEGF secretion in ARPE-19 human retinal pigment epithelium cells. Curr Eye Res 34:57-1 CrossRef
  6. Ambati J, Anand A, Fernandez S, Sakurai E, Lynn BC, Kuziel WA, Rollins BJ, Ambati BK (2003) An animal model of age-related macular degeneration in senescent Ccl-2- or Ccr-2-deficient mice. Nat Med 9:1390-397 CrossRef
  7. Fukuoka Y, Strainic M, Medof ME (2003) Differential cytokine expression of human retinal pigment epithelial cells in response to stimulation by C5a. Clin Exp Immunol 131:248-53 CrossRef
  8. Wakabayashi Y, Usui Y, Okunuki Y, Takeuchi M, Kezuka T, Iwasaki T, Goto H (2008) Increased levels of monokine induced by interferon-gamma (Mig) in the vitreous of patients with diabetic retinopathy. Diabet Med 25:875-77 CrossRef
  9. Wakabayashi Y, Usui Y, Okunuki Y, Kezuka T, Takeuchi M, Iwasaki T, Ohno A, Goto H (2011) Increases of vitreous monocyte chemotactic protein 1 and interleukin 8 levels in patients with concurrent hypertension and diabetic retinopathy. Retina 31:1951-957 CrossRef
  
• 作者单位：Daisuke Muramatsu (1)
  Yoshihiro Wakabayashi (1)
  Yoshihiko Usui (1)
  Yoko Okunuki (1)
  Takeshi Kezuka (1)
  Hiroshi Goto (1)
  
  1. Department of Ophthalmology, Tokyo Medical University, 6-7-1, Nishishinjuku, Shinjuku-ku, Tokyo, 160-0023, Japan
  
• ISSN：1435-702X

文摘

Purpose To determine the vitreous concentration of complement fragment C5a in patients with proliferative diabetic retinopathy (PDR) and the relation between C5a and inflammatory cytokines including vascular endothelial growth factor (VEGF) and monocyte chemotactic protein-1 (MCP-1). Methods Vitreous samples were obtained at the time of vitrectomy from 12 eyes of 11 PDR patients and from 11 eyes of 11 patients without diabetes with macular disease (controls). Vitreous and serum concentrations of human C5a, VEGF, and MCP-1 were quantified using FACS Caliber? flow cytometer. Results Vitreous concentration of C5a increased significantly in patients with PDR [median (range): 928.7 (46.6 to 3,319.4) pg/ml] compared with controls [58.7 (22.2 to 1,432.4) pg/ml; p-lt;-.01]. In PDR patients, vitreous concentration of C5a correlated significantly with those of VEGF (p-lt;-.05) and MCP-1 (p-lt;-.05). Conclusions Our results suggest that C5a may play an important role in the pathogenesis of PDR and work in concert with inflammatory cytokines such as VEGF and MCP-1 in pathological angiogenesis.