Molecular Cloning and Sequence Analysis of the Phosphoprotein (P) Gene of the Lapinized Rinderpest Virus

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• 作者：Kazuya Nakamura ; Kiyoko Iwatsuki ; Yoshihiro Izumiya ; Yorihiro Nishimura ; Kenjiro Ohashi ; Takeshi Mikami and Chieko Kai
• 关键词：rinderpest virus ; lapinized strain ; P gene
• 刊名：Virus Genes
• 出版年：1999
• 出版时间：1999
• 年：1999
• 卷：18
• 期：2
• 页码：175-178
• 全文大小：95 KB

文摘

We determined the nucleotide sequence of the coding region for the phosphoprotein (P) gene of the L strain of rinderpest virus (RPV). The gene encodes two overlapping open reading frames of 1521 and 531 nucleotides. Use of the first ATG would produce a P polypeptide of 507 amino acids, while use of the second ATG would produce a C polypeptide of 177 amino acids. In addition, the insertion of an extra G residue at the editing site generates an alternative mRNA potentially encoding the V protein of RPV. Homology comparisons of the P, C and V proteins among various viruses suggest that RPV is closer to measles virus (MV) than to canine distemper virus (CDV). Alignment of the sequences unique to the V protein revealed that the cysteine residues are well conserved among RPV, MV and CDV, and form a zinc finger-like motif.