Transcriptomic, proteomic and metabolic changes in Arabidopsis thaliana leaves after the onset of illumination

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• 作者：Chao Liang ; Shifeng Cheng ; Youjun Zhang ; Yuzhe Sun ; Alisdair R. Fernie…
• 关键词：ATP ; Chloroplast ; Mitochondria ; Metabolomics ; Proteomics ; Transcriptomics
• 刊名：BMC Plant Biology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：16
• 期：1
• 全文大小：3,088 KB
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• 作者单位：Chao Liang (1)
  Shifeng Cheng (1)
  Youjun Zhang (2)
  Yuzhe Sun (1)
  Alisdair R. Fernie (2)
  Kang Kang (1)
  Gianni Panagiotou (1)
  Clive Lo (1)
  Boon Leong Lim (1) (3)
  
  1. School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China
  2. Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany
  3. State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong, China
  
• 刊物主题：Plant Sciences; Agriculture; Tree Biology;
• 出版者：BioMed Central
• ISSN：1471-2229

文摘

Background Light plays an important role in plant growth and development. In this study, the impact of light on physiology of 20-d-old Arabidopsis leaves was examined through transcriptomic, proteomic and metabolomic analysis. Since the energy-generating electron transport chains in chloroplasts and mitochondria are encoded by both nuclear and organellar genomes, sequencing total RNA after removal of ribosomal RNAs provides essential information on transcription of organellar genomes. The changes in the levels of ADP, ATP, NADP+, NADPH and 41 metabolites upon illumination were also quantified.