Development of a fluorescent probe-based recombinase polymerase amplification assay for rapid detection of Orf virus
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- 作者：Yang Yang ; Xiaodong Qin ; Guangxiang Wang ; Yuen Zhang ; Youjun Shang…
- 关键词：Recombinase polymerase amplification assay ; RPA assay ; Orf virus ; Small ruminants
- 刊名：Virology Journal
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：12
- 期：1
- 全文大小：601 KB
- 刊物主题：Virology;
- 出版者：BioMed Central
- ISSN：1743-422X
文摘
Background Orf virus (ORFV) is the causative agent of Orf (also known as contagious ecthyma or contagious papular dermatitis), a severe infectious skin disease in goats, sheep and other ruminants. The rapid detection of ORFV is of great importance in disease control and highly needed. A isothermal molecular diagnostic approach, termed recombinase polymerase amplification (RPA), is considered as an novel and rapid alternative techonology to PCR assay.