Comparison of phbC genes cloned from Ralstonia eutropha and Alcaligenes latus for utilization in metabolic engineering of polyhydroxyalkanoate biosynthesis
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- 作者：Shin ; Hyun Dong ; Oh ; Dong Hyun ; Jung ; Young Mi ; Ghim ; Sa Youl ; Lee ; Yong Hyun
- 关键词：Alcaligenes latus ; metabolic engineering ; phbC gene ; polyhydroxy ; alkanoates ; Ralstonia eutropha
- 刊名：Biotechnology Letters
- 出版年：2002
- 出版时间：2002
- 年：2002
- 卷：24
- 期：7
- 页码：539-545
- 全文大小：115 KB
文摘
Two cloned phbC genes, encoding polyhydroxybutyrate (PHB) synthase from Ralstonia eutropha and from Alcaligenes latus, were transformed into a PHB-negative mutant of R. eutropha. The expression characteristics of both genes were compared for the biosyntheses of PHB and its copolymers. Each phbC gene had different characteristics not only in the biosyntheses of PHB, poly(3-hydroxybutyrate-3-hydroxy-valerate), and poly(3-hydroxybutyrate-4-hydroxybutyrate) but also in the resulting morphology of PHB granules.