Constitutive expression of CaPLA1 conferred enhanced growth and grain yield in transgenic rice plants

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• 作者：Ki Youl Park ; Eun Yu Kim ; Young Sam Seo ; Woo Taek Kim
• 关键词：Enhanced growth ; Grain yield ; Transgenic rice plant ; Membrane peroxidation ; Phospholipase A1 ; Oryza sativa
• 刊名：Plant Molecular Biology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：90
• 期：4-5
• 页码：517-532
• 全文大小：3,336 KB
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• 作者单位：Ki Youl Park (1)
  Eun Yu Kim (1)
  Young Sam Seo (1) (2)
  Woo Taek Kim (1)
  
  1. Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, Korea
  2. Research Institute, Korea Ginseng Corp., Daejeon, 305-805, Korea
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Biochemistry
  Plant Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-5028

文摘

Phospholipids are not only important components of cell membranes, but participate in diverse processes in higher plants. In this study, we generated Capsicum annuum phospholipiase A1 (CaPLA1) overexpressing transgenic rice (Oryza sativa L.) plants under the control of the maize ubiquitin promoter. The T4 CaPLA1-overexpressing rice plants (Ubi:CaPLA1) had a higher root:shoot mass ratio than the wild-type plants in the vegetative stage. Leaf epidermal cells from transgenic plants had more cells than wild-type plants. Genes that code for cyclin and lipid metabolic enzymes were up-regulated in the transgenic lines. When grown under typical paddy field conditions, the transgenic plants produced more tillers, longer panicles and more branches per panicle than the wild-type plants, all of which resulted in greater grain yield. Microarray analysis suggests that gene expressions that are related with cell proliferation, lipid metabolism, and redox state were widely altered in CaPLA1-overexpressing transgenic rice plants. Ubi:CaPLA1 plants had a reduced membrane peroxidation state, as determined by malondialdehyde and conjugated diene levels and higher peroxidase activity than wild-type rice plants. Furthermore, three isoprenoid synthetic genes encoding terpenoid synthase, hydroxysteroid dehydrogenase and 3-hydroxy-3-methyl-glutaryl-CoA reductase were up-regulated in CaPLA1-overexpressing plants. We suggest that constitutive expression of CaPLA1 conferred increased grain yield with enhanced growth in transgenic rice plants by alteration of gene activities related with cell proliferation, lipid metabolism, membrane peroxidation state and isoprenoid biosynthesis.