Optimization of factors affecting efficient shoot regeneration in chrysanthemum cv. Shinma

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• 作者：Aung Htay Naing ; Kyeung Il Park ; Mi Young Chung…
• 关键词：Dark treatment ; Donor plant age ; Ethylene inhibitors ; Flow cytometry ; Ornamental plant ; Plant growth regulator
• 刊名：Brazilian Journal of Botany
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：39
• 期：4
• 页码：975-984
• 全文大小：
• 刊物主题：Plant Systematics/Taxonomy/Biogeography;
• 出版者：Springer International Publishing
• ISSN：1806-9959
• 卷排序：39

文摘

Factors affecting shoot regeneration were optimized in order to develop an efficient protocol for shoot regeneration in leaf explants of the chrysanthemum (Chrysanthemum morifolium Ramat) cultivar Shinma. The maximum number of shoots per explant (9.7) was regenerated in leaf explants of 6-week-old donor plants cultured on Murashige–Skoog medium containing a combination of 0.5 mg L−1 6-benzyladenine and 0.5 mg L−1 α-naphthaleneacetic acid with initial dark treatment for 7 days. Among the different auxins tested, indolebutyric acid was the most effective for root induction and development, whereas the presence of ethylene inhibitors strongly suppressed shoot regeneration. Ploidy levels were analyzed by flow cytometry: there was no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions. The new protocol will facilitate genetic transformation and micropropagation of chrysanthemum cv. Shinma.