Enhancing the activity of Bacillus circulans xylanase by modulating the flexibility of the hinge region
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- 作者：Fukura Kazuyo (1)
So Yeon Hong (1)
Young Joo Yeon (2)
Jeong Chan Joo (2) (4)
Young Je Yoo (1) (2) (3) - 关键词：Xylanase ; Activity ; Dynamics ; Flexibility ; Hinge
- 刊名：Journal of Industrial Microbiology and Biotechnology
- 出版年：2014
- 出版时间：August 2014
- 年：2014
- 卷：41
- 期：8
- 页码：1181-1190
- 全文大小：775 KB
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So Yeon Hong (1)
Young Joo Yeon (2)
Jeong Chan Joo (2) (4)
Young Je Yoo (1) (2) (3)
1. Graduate Program of Bioengineering, Seoul National University, Seoul, 151-742, Republic of Korea
2. School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, Republic of Korea
4. Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, M5S 3E5, Canada
3. Bio-MAX Institute, Seoul National University, Seoul, 151-742, Republic of Korea - ISSN：1476-5535
文摘
Enzymes undergo multiple conformational changes in solution, and these dynamics are considered to play a critical role in enzyme activity. Hinge-bending motions, resulting from reciprocal movements of dynamical quasi-rigid bodies, are thought to be related to turnover rate and are affected by the physical properties of the hinge regions. In this study, hinge identification and flexibility modification of the regions by mutagenesis were conducted to explore the relationship between hinge flexibility and catalytic activity. Bacillus circulans xylanase was selected for the identification and mutation of the hinge regions. As a result, turnover rate (V max) was improved approximately twofold in mutants that have more rigid hinge structure, despite the decrease in K m and V max/K m. This result indicates that the rigidly mutated hinge has positive effects on B. circulans xylanase activity.