Biosynthesis of pinene from glucose using metabolically-engineered Corynebacterium glutamicum

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• 作者：Min-Kyoung Kang ; Jin-Hee Eom ; Yunje Kim ; Youngsoon Um ; Han Min Woo
• 关键词：Corynebacterium Glutamicum ; Metabolic Engineering ; Monoterpene ; Pinene ; Toxicity
• 刊名：Biotechnology Letters
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：36
• 期：10
• 页码：2069-2077
• 全文大小：765 KB
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• 作者单位：Min-Kyoung Kang (1)
  Jin-Hee Eom (1) (2)
  Yunje Kim (1)
  Youngsoon Um (1) (4)
  Han Min Woo (1) (3) (4)
  
  1. Clean Energy Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5 Seongbuk-gu, Seoul, 136-791, Republic of Korea
  2. Department of Chemistry, Korea University, 145 Anam-ro Seongbuk-gu, Seoul, 136-701, Republic of Korea
  4. Department of Clean Energy and Chemical Engineering, Korea University of Science and Technology, 217 Gajeong-ro Yuseong-gu, Daejeon, 305-350, Republic of Korea
  3. Green School, Korea University, 145 Anam-ro Seongbuk-gu, Seoul, 136-701, Republic of Korea
  
• ISSN：1573-6776

文摘

Pinene is a monoterpenes (C10) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. Herein, we have metabolically-engineered Corynebacterium glutamicum, to produce pinene and studied its toxicity in C. glutamicum. Geranyl diphosphate synthases (GPPS) and pinene synthases (PS), obtained from Pinus taeda and Abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Idi) from C. glutamicum using CoryneBrick vector. Most strains expressing PS-GPPSs produced detectable amounts of pinene, but co-expression of DXS and IDI with PS (P. taeda) and GPPS (A. grandis) resulted in 27?μg?±?7 α-pinene g??cell dry weight, which is the first report in C. glutamicum. Further engineering of PS and GPPS in the C. glutamicum strain may increase pinene production.