Adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative Corynebacterium glutamicum that co-utilizes cellobiose and xylose
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- 作者：Jungseok Lee ; Jack N. Saddler ; Youngsoon Um ; Han Min Woo
- 关键词：Corynebacterium glutamicum ; Cellobiose and xylose ; Cofermentation ; Intracellular β ; glucosidase ; Adaptive evolution
- 刊名：Microbial Cell Factories
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：15
- 期：1
- 全文大小：3,726 KB
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Jack N. Saddler (2)
Youngsoon Um (1) (3)
Han Min Woo (1) (3) (4)
1. Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Hwarangro 14-gil 5, Seongbuk-gu, Seoul, 02792, Republic of Korea
2. Department of Wood Science, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada
3. Department of Clean Energy and Chemical Engineering, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea
4. Green School (Graduate School of Energy and Environment), Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea - 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology
Applied Microbiology
Environmental Engineering/Biotechnology - 出版者：BioMed Central
- ISSN：1475-2859
文摘
Background An efficient microbial cell factory requires a microorganism that can utilize a broad range of substrates to economically produce value-added chemicals and fuels. The industrially important bacterium Corynebacterium glutamicum has been studied to broaden substrate utilizations for lignocellulose-derived sugars. However, C. glutamicum ATCC 13032 is incapable of PTS-dependent utilization of cellobiose because it has missing genes annotated to β-glucosidases (bG) and cellobiose-specific PTS permease.