Conversion of levulinic acid to 2-butanone by acetoacetate decarboxylase from Clostridium acetobutylicum
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- 作者：Kyoungseon Min (1)
Seil Kim (1)
Taewoo Yum (1)
Yunje Kim (1)
Byoung-In Sang (2)
Youngsoon Um (1) - 关键词：Enzymatic decarboxylation ; Acetoacetate decarboxylase ; Levulinic acid ; 2 ; Butanone
- 刊名：Applied Microbiology and Biotechnology
- 出版年：2013
- 出版时间：June 2013
- 年：2013
- 卷：97
- 期：12
- 页码：5627-5634
- 全文大小：300KB
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Seil Kim (1)
Taewoo Yum (1)
Yunje Kim (1)
Byoung-In Sang (2)
Youngsoon Um (1)
1. Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, 136-791, South Korea
2. Department of Chemical Engineering, Hanyang University, Seoul, 133-791, South Korea - ISSN：1432-0614
文摘
In this study, a novel system for synthesis of 2-butanone from levulinic acid (γ-keto-acid) via an enzymatic reaction was developed. Acetoacetate decarboxylase (AADC; E.C. 4.1.1.4) from Clostridium acetobutylicum was selected as a biocatalyst for decarboxylation of levulinic acid. The purified recombinant AADC from Escherichia coli successfully converted levulinic acid to 2-butanone with a conversion yield of 8.4-0.3?% depending on the amount of AADC under optimum conditions (30?°C and pH?5.0) despite that acetoacetate, a β-keto-acid, is a natural substrate of AADC. In order to improve the catalytic efficiency, an AADC-mediator system was tested using methyl viologen, methylene blue, azure B, zinc ion, and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as mediators. Among them, methyl viologen showed the best performance, increasing the conversion yield up to 6.7-fold in comparison to that without methyl viologen. The results in this study are significant in the development of a renewable method for the synthesis of 2-butanone from biomass-derived chemical, levulinic acid, through enzymatic decarboxylation.