Selective Production of 2,3-Butanediol and Acetoin by a Newly Isolated Bacterium Klebsiella oxytoca M1

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• 作者：Sukhyeong Cho (1)
  Kyung Duk Kim (1)
  Jae-Hyung Ahn (2)
  Jinwon Lee (3)
  Seon-Won Kim (4)
  Youngsoon Um (1)
  
• 关键词：Klebsiella oxytoca ; 2 ; 3 ; Butanediol ; Acetoin
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2013
• 出版时间：August 2013
• 年：2013
• 卷：170
• 期：8
• 页码：1922-1933
• 全文大小：257KB
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• 作者单位：Sukhyeong Cho (1)
  Kyung Duk Kim (1)
  Jae-Hyung Ahn (2)
  Jinwon Lee (3)
  Seon-Won Kim (4)
  Youngsoon Um (1)
  
  1. Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, 136-791, Republic of Korea
  2. Agricultural Microbiology Division, Rural Development Administration, 54 Seohoro, Gwonseon-gu, Suwon, Gyeonggi-do, 441-853, Republic of Korea
  3. Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, Republic of Korea
  4. Division of Applied Life Sciences (BK21), PMBBRC, Gyeongsang National University, Jinju, Gyeongnam, 660-701, Republic of Korea
  

文摘

A newly isolated bacterium, designated as Klebsiella oxytoca M1, produced 2,3-butanediol (2,3-BDO) or acetoin selectively as a major product depending on temperature in a defined medium. K. oxytoca M1 produced 2,3-BDO mainly (0.32~0.34?g/g glucose) at 30?°C while acetoin was a major product (0.32~0.38?g/g glucose) at 37?°C. To investigate factors affecting product profiles according to temperature, the expression level of acetoin reductase (AR) that catalyzes the conversion of acetoin to 2,3-BDO was analyzed using crude protein extracted from K. oxytoca M1 grown at 30 and 37?°C. The AR expression at 37?°C was 12.8-fold lower than that at 30?°C at the stationary phase and reverse transcription PCR (RT-PCR) analysis of the budC (encoding AR) was also in agreement with the AR expression results. When AR was overexpressed using K. oxytoca M1 harboring pUC18CM-budC, 2,3-BDO became a major product at 37?°C, indicating that the AR expression level was a key factor determining the major product of K. oxytoca M1 at 37?°C. The results in this study demonstrate the feasibility of using K. oxytoca M1 for the production of not only 2,3-BDO but also acetoin as a major product.