Effects of low-intensity pulsed ultrasound and hyperbaric oxygen on human osteoarthritic chondrocytes

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• 作者：Li-Jen Yuan (1)
  Chi-Chien Niu (1)
  Song-Shu Lin (1) (2)
  Chuen-Yung Yang (1)
  Yi-Sheng Chan (1)
  Wen-Jer Chen (1)
  Steve WN Ueng (1)
  
• 关键词：Low ; intensity pulsed ultrasound ; Hyperbaric oxygen ; Osteoarthritis ; TIMP ; 1 ; MMP ; 3 ; Aggrecan ; Type ; II collagen
• 刊名：Journal of Orthopaedic Surgery and Research
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：9
• 期：1
• 全文大小：243 KB
• 作者单位：Li-Jen Yuan (1)
  Chi-Chien Niu (1)
  Song-Shu Lin (1) (2)
  Chuen-Yung Yang (1)
  Yi-Sheng Chan (1)
  Wen-Jer Chen (1)
  Steve WN Ueng (1)
  
  1. Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, 5, Fu-Hsin St. 333, Kweishan, Taoyuan, 333, Taiwan
  2. Graduate Institute of Biomedical Sciences, Chang Gung University, 259 Wen-Hwa 1st Road, Taoyuan, 333, Taiwan
  
• ISSN：1749-799X

文摘

Background Although the individual effects of hyperbaric oxygen (HBO) and low-intensity pulsed ultrasound (LIPUS) on osteoarthritic (OA) chondrocytes have been reported, the effects of HBO combined with LIPUS treatment are unknown. Methods OA chondrocytes were obtained from patients undergoing knee replacement surgery. RNA was isolated for real-time polymerase chain reaction (PCR) analysis of inducible nitric oxide synthase (iNOS), type-II collagen, and aggrecan gene expression. The protein levels of MMP-3 and TIMP-1 were quantified by enzyme-linked immunosorbent assay (ELISA) after LIPUS or HBO treatment. The data are given as mean?±?standard deviation (SD) of the results from three independent experiments. A p value less than 0.05 was defined as statistically significant. Results Our data suggested that ultrasound and HBO treatment increased cell bioactivity of OA chondrocytes. Real-time PCR analysis showed that HBO treatment increased the mRNA of type-II collagen, aggrecan, and TIMP-1 but suppressed the iNOS expression of OA chondrocytes. LIPUS treatment increased the type-II collagen and iNOS expression of OA chondrocytes. ELISA data showed that HBO or LIPUS treatment increased TIMP-1 production of OA chondrocyte. MMP-3 production was suppressed by HBO treatment. HBO combined with LIPUS treatments resulted in additive effect in TIMP-1 production and compensatory effect in iNOS expression. Conclusion HBO combined with LIPUS treatment-induced increase of the anabolic factor (TIMP-1)/catabolic factor (MMP-3) ratio may provide an additive therapeutic approach to slow the course of OA degeneration.