Nucleotidyl transferase TUT1 inhibits lipogenesis in osteosarcoma cells through regulation of microRNA-24 and microRNA-29a

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• 作者：De-qiu Zhu (1)
  Yue-fen Lou (2)
  Zhi-gao He (3)
  Min Ji (4)
  
• 关键词：Osteosarcoma cells ; MicroRNA ; 24 ; TUT1 ; MicroRNA ; 29a
• 刊名：Tumor Biology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：35
• 期：12
• 页码：11829-11835
• 全文大小：701 KB
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• 作者单位：De-qiu Zhu (1)
  Yue-fen Lou (2)
  Zhi-gao He (3)
  Min Ji (4)
  
  1. Department of Pharmacy, Shanghai Tongji Hospital, 200065, Shanghai, China
  2. Department of Pharmacy, The Branch of the First People鈥檚 Hospital of Shanghai City (the fourth hospital), 200081, Shanghai, China
  3. Department of Pharmacy, East Hospital, Tongji University School of Medicine, 200120, Shanghai, China
  4. Department of Pharmacy, Yangpu Hospital, Tongji University School of Medicine, 200090, Shanghai, China
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

Osteosarcoma is the most common type of bone cancer. In the present study, by way of PCR-based microarrays, we found that TUT1, a nucleotidyl transferase, was significantly downregulated in osteosarcoma, compared with adjacent normal tissues. In the current study, we performed PCR-based microarrays using the cDNA prepared from osteosarcoma and adjacent normal tissues. The enforced expression of TUT1 was able to inhibit cell proliferation in U2OS and MG63 cells, while its knockdown using small interfering RNA (siRNA) oligos promoted cell proliferation. At the molecular level, we found that TUT1 could inhibit the expression levels of PPARgamma and SREBP-1c, two key regulators in lipogenesis, through upregulation of microRNA-24 and microRNA-29a. Therefore, our results suggest that TUT1 may act as a tumor suppressor for osteosarcoma, which might provide a novel mechanism for the tumor development.