Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix
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- 作者：Yi Shao (1) (2) (4)
Luowa Quyang (2)
Yueping Zhou (2)
Jing Tang (2) (3)
Yuehui Tan (2)
Qiuping Liu (2)
Zhirong Lin (1) (2)
Tingting Yin (2)
Fangfang Qiu (2)
Zuguo Liu (2) (5) - 关键词：Cornea matrix ; Human sera ; Tissue engineering ; Decellularization biocompatibily
- 刊名：In Vitro Cellular & Developmental Biology - Animal
- 出版年：2010
- 出版时间：July 2010
- 年：2010
- 卷：46
- 期：7
- 页码：600-605
- 全文大小：384KB
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Luowa Quyang (2)
Yueping Zhou (2)
Jing Tang (2) (3)
Yuehui Tan (2)
Qiuping Liu (2)
Zhirong Lin (1) (2)
Tingting Yin (2)
Fangfang Qiu (2)
Zuguo Liu (2) (5)
1. Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology of Sun Yat-sen University, Guangzhou, Guangdong Province, China
2. Fujian Province Key Laboratory of Ophthalmology and Vision Research, Eye Institute of Xiamen University, Xiamen, Fujian Province, China
4. The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China
3. Affiliated Zhongshan Hospital of Xiamen University, Xiamen, Fujian Province, China
5. Eye Institute of Xiamen University, No. 168 Da Xue Road, Xiamen, 361005, People’s Republic of China
文摘
This study was to investigate the stability, physico-mechanical property and biocompatibility of porcine corneal acellularized matrix (PCAM) that was prepared using human sera treatment to decellularize corneas. The stability (the rate of biodegradation) and physico-mechanical property (water uptake, density, and porosity) of PCAM were not compromised, compared with porcine fresh cornea matrix (PFCM, p-gt;-.05). The contact and extract cytotoxicity tests with human corneal epithelial cells and human keratocytes showed that PCAM has a good biocompatibility ex vivo and no cytotoxic effect. These results present the ability to create safety scaffolds that function as cornea grafts and provide a novel experimental approach for the study of cornea tissue engineering using acellular porcine cornea.