Development and characterization of microsatellite loci in Opisthopappus taihangensis (Compositae), a rare herb endemic to China
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- 作者：Songyu Pei (1)
Yueqin Cheng (1)
Yuyao Wang (1)
Zhenbin Jiao (1)
Hongwei Wang (1)
1. College of Plant Protection ; Henan Agricultural University ; Zhengzhou ; 450002 ; China - 关键词：Microsatellite ; Genetic conservation ; Opisthopappus taihangensis
- 刊名：Conservation Genetics Resources
- 出版年：2015
- 出版时间：March 2015
- 年：2015
- 卷：7
- 期：1
- 页码：269-271
- 全文大小：153 KB
- 参考文献：1. Ding BZ, Wang SY (1998) Flora of Henan. Henan Science and Technology Press, Zhengzhou, p 632
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- 出版者：Springer Netherlands
- ISSN：1877-7260
文摘
Using an enriched genomic library, we developed and characterized 10 polymorphic microsatellite primers for Opisthopappus taihangensis, a rare herb endemic to China. Number of alleles varied from 2 to 8 per locus, observed and expected heterozygosities ranged from 0.214 to 0.929 and 0.262 to 0.746 respectively. Significantly deviations from HWE were detected in two loci. These microsatellite loci would be useful for studies of genetic variation, genetic structure, and mating systems of O. taihangensis, which are important for the further conservation of this rare species.