Development of a direct competitive ELISA for the detection of Mycoplasma bovis infection based on a monoclonal antibody of P48 protein
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- 作者：Ping Fu (1)
Zhenhong Sun (1)
Yuewei Zhang (1)
Ziqiang Yu (1)
Haiyan Zhang (1)
Dan Su (2)
Fei Jiang (1)
Wenxue Wu (1) - 关键词：Direct competitive ELISA ; Mycoplasma bovis ; Monoclonal antibody
- 刊名：BMC Veterinary Research
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：10
- 期：1
- 全文大小：306 KB
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Zhenhong Sun (1)
Yuewei Zhang (1)
Ziqiang Yu (1)
Haiyan Zhang (1)
Dan Su (2)
Fei Jiang (1)
Wenxue Wu (1)
1. Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
2. Shandong Vocational Animal Science and Veterinary College, Weifang, China - ISSN：1746-6148
文摘
Background Mycoplasma bovis (M. bovis) is a major, but often overlooked, pathogen documented to cause respiratory disease, mastitis, and arthritis in cattle throughout China since 2008. Here, we report the development of a direct competitive enzyme-linked immunosorbent assay (Dc-ELISA) to detect M. bovis antibody. Results We used a recombinant P48 protein and monoclonal antibody (mAb) 10E. MAb 10E, prepared against the recombinant P48 protein of M. bovis, identified all M. bovis strains with no cross-reactivity with other related pathogens. Coating micro plates with P48 protein instead of whole M. bovis cells as well as the use of mAb 10E produced a specific and sensitive Dc-ELISA for M. bovis antibody detection with a cut-off percent inhibition (PI) value of 32%. Compared with two commercial indirect ELISA (i-ELISA) kits, our Dc-ELISA offered a higher positive detection rate in 165 clinical bovine serum samples. Conclusions A rapid, sensitive, and reliable serological diagnosis method was developed for M. bovis, which can facilitate M. bovis surveillance, assisting researchers in understanding the ecology and epidemiology of M. bovis.