Upregulated Expression of SSTR1 is Involved in Neuronal Apoptosis and is Coupled to the Reduction of bcl-2 Following Intracerebral Hemorrhage in Adult Rats

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• 作者：Damin Yuan (1)
  Jianhong Shen (2)
  Yaohua Yan (2)
  Xinmin Wu (2)
  Aihong Li (2)
  Aisong Guo (2)
  Yuanyuan Wu (2)
  Chengwei Duan (3)
  Jiabing Shen (4)
  Cuiying Tang (5)
  Dongmei Zhang (6)
  Yuhong Ji (1)
  
• 关键词：Intracerebral hemorrhage ; SSTR1 ; Neuron ; Apoptosis
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：34
• 期：7
• 页码：951-961
• 全文大小：2,774 KB
• 参考文献：1. Akopian A, Johnson J, Gabriel R, Brecha N, Witkovsky P (2000) Somatostatin modulates voltage-gated K(+) and Ca(2+) currents in rod and cone photoreceptors of the salamander retina. J Neurosci 20:929鈥?36
  2. Aronowski J, Zhao X (2011) Molecular pathophysiology of cerebral hemorrhage: secondary brain injury. Stroke 42:1781鈥?786 CrossRef
  3. Bradl M, Lassmann H (2010) Oligodendrocytes: biology and pathology. Acta Neuropathol 119:37鈥?3 CrossRef
  4. Broughton BR, Reutens DC, Sobey CG (2009) Apoptotic mechanisms after cerebral ischemia. Stroke 40:e331鈥揺339 CrossRef
  5. Chatterjee S et al (2007) Prolonged somatostatin therapy may cause down-regulation of SSTR-like GPCRs on Schistosoma mansoni. J Vector Borne Dis 44:164鈥?80
  6. Cregan SP, MacLaurin JG, Craig CG, Robertson GS, Nicholson DW, Park DS, Slack RS (1999) Bax-dependent caspase-3 activation is a key determinant in p53-induced apoptosis in neurons. J Neurosci 19:7860鈥?869
  7. Epelbaum J, Dournaud P, Fodor M, Viollet C (1994) The neurobiology of somatostatin. Crit Rev Neurobiol 8:25鈥?4
  8. Hu C et al (2004) The effect of somatostatin and SSTR3 on proliferation and apoptosis of gastric cancer cells. Cancer Biol Ther 3:726鈥?30 CrossRef
  9. Hua Y, Schallert T, Keep RF, Wu J, Hoff JT, Xi G (2002) Behavioral tests after intracerebral hemorrhage in the rat. Stroke 33:2478鈥?484 CrossRef
  10. Huan W et al (2012) Spatiotemporal patterns and essential role of TNF receptor-associated factor 5 expression after rat spinal cord Injury. J Mol Histol 43:527鈥?33 CrossRef
  11. Ikram MA, Wieberdink RG, Koudstaal PJ (2012) International epidemiology of intracerebral hemorrhage. Curr Atheroscler Rep 14:300鈥?06 CrossRef
  12. Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H (2014) Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways. Int J Mol Med 33:1298鈥?304
  13. Karabiyikoglu M, Hua Y, Keep RF, Ennis SR, Xi G (2004) Intracerebral hirudin injection attenuates ischemic damage and neurologic deficits without altering local cerebral blood flow. J Cereb Blood Flow Metab 24:159鈥?66 CrossRef
  14. Karwacki Z et al (2005) Apoptosis in the course of experimental intracerebral haemorrhage in the rat. Folia Morphol (Warsz) 64:248鈥?52
  15. Kroemer G, Galluzzi L, Brenner C (2007) Mitochondrial membrane permeabilization in cell death. Physiol Rev 87:99鈥?63 CrossRef
  16. Li L et al (2013) Up-regulation of NFATc4 involves in neuronal apoptosis following intracerebral hemorrhage. Cell Mol Neurobiol 33:893鈥?05 CrossRef
  17. Liu X, Kim CN, Yang J, Jemmerson R, Wang X (1996) Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell 86:147鈥?57 CrossRef
  18. Liu X, Yuan D, Nie X, Shen J, Yan Y, Zhang D, Gu J (2014) BTEB2 prevents neuronal apoptosis via promoting bad phosphorylation in rat intracerebral hemorrhage model. J Mol Neurosci
  19. Martinou JC, Youle RJ (2011) Mitochondria in apoptosis: bcl-2 family members and mitochondrial dynamics. Dev Cell 21:92鈥?01 CrossRef
  20. Papadaki T, Tsilimbaris M, Pallikaris I, Thermos K (2010) Somatostatin receptor activation (sst(1) -sst(5)) differentially influences human retinal pigment epithelium cell viability. Acta Ophthalmol 88:e228鈥揺233 CrossRef
  21. Patel YC (1999) Somatostatin and its receptor family. Front Neuroendocrinol 20:157鈥?98 CrossRef
  22. Patel YC, Srikant CB (1994) Subtype selectivity of peptide analogs for all five cloned human somatostatin receptors (hsstr 1-5). Endocrinology 135:2814鈥?817
  23. Qiu CZ, Wang C, Huang ZX, Zhu SZ, Wu YY, Qiu JL (2006) Relationship between somatostatin receptor subtype expression and clinicopathology, Ki-67, bcl-2 and p53 in colorectal cancer. World J Gastroenterol 12:2011鈥?015
  24. Qureshi AI, Mendelow AD, Hanley DF (2009) Intracerebral haemorrhage. Lancet 373:1632鈥?644 CrossRef
  25. Reubi JC (1992) Somatostatin receptors in the gastrointestinal tract in health and disease. Yale J Biol Med 65:493鈥?03 discussion 531-496
  26. Rochaix P et al (1999) Gene therapy for pancreatic carcinoma: local and distant antitumor effects after somatostatin receptor sst2 gene transfer. Hum Gene Ther 10:995鈥?008 CrossRef
  27. Rui Y et al (2013) Up-regulated expression of Bnip3L after intracerebral hemorrhage in adult rats. J Mol Histol 44:497鈥?05 CrossRef
  28. Sharma K, Srikant CB (1998) G protein coupled receptor signaled apoptosis is associated with activation of a cation insensitive acidic endonuclease and intracellular acidification. Biochem Biophys Res Commun 242:134鈥?40 CrossRef
  29. Sharma K, Patel YC, Srikant CB (1996) Subtype-selective induction of wild-type p53 and apoptosis, but not cell cycle arrest, by human somatostatin receptor 3. Mol Endocrinol 10:1688鈥?696
  30. Stetak A, Lankenau A, Vantus T, Csermely P, Ullrich A, Keri G (2001) The antitumor somatostatin analogue TT-232 induces cell cycle arrest through PKCdelta and c-Src. Biochem Biophys Res Commun 285:483鈥?88 CrossRef
  31. Teijeiro R, Rios R, Costoya JA, Castro R, Bello JL, Devesa J, Arce VM (2002) Activation of human somatostatin receptor 2 promotes apoptosis through a mechanism that is independent from induction of p53. Cell Physiol Biochem 12:31鈥?8 CrossRef
  32. Tsai CF, Thomas B, Sudlow CL (2013) Epidemiology of stroke and its subtypes in Chinese vs white populations: a systematic review. Neurology 81:264鈥?72 CrossRef
  33. Vaux DL (2011) Apoptogenic factors released from mitochondria. Biochim Biophys Acta 1813:546鈥?50 CrossRef
  34. Wang J, Dore S (2007) Inflammation after intracerebral hemorrhage. J Cereb Blood Flow Metab 27:894鈥?08 CrossRef
  35. War SA, Kumar U (2012) Coexpression of human somatostatin receptor-2 (SSTR2) and SSTR3 modulates antiproliferative signaling and apoptosis. J Mol Signal 7:5 CrossRef
  36. War SA, Somvanshi RK, Kumar U (2011) Somatostatin receptor-3 mediated intracellular signaling and apoptosis is regulated by its cytoplasmic terminal. Biochim Biophys Acta 1813:390鈥?02 CrossRef
  37. Watt HL, Kharmate GD, Kumar U (2009) Somatostatin receptors 1 and 5 heterodimerize with epidermal growth factor receptor: agonist-dependent modulation of the downstream MAPK signalling pathway in breast cancer cells. Cell Signal 21:428鈥?39 CrossRef
  38. Xi G, Fewel ME, Hua Y, Thompson BG Jr, Hoff JT, Keep RF (2004) Intracerebral hemorrhage: pathophysiology and therapy. Neurocrit Care 1:5鈥?8 CrossRef
  39. Xue M, Del Bigio MR (2003) Comparison of brain cell death and inflammatory reaction in three models of intracerebral hemorrhage in adult rats. J Stroke Cerebrovasc Dis 12:152鈥?59 CrossRef
  40. Yoon JH, Lee HH, Yi ES, Baek SG (2013) Age-dependent effect of treadmill exercise on hemorrhage-induced neuronal cell death in rats. J Exerc Rehabil 9:506鈥?10 CrossRef
  
• 作者单位：Damin Yuan (1)
  Jianhong Shen (2)
  Yaohua Yan (2)
  Xinmin Wu (2)
  Aihong Li (2)
  Aisong Guo (2)
  Yuanyuan Wu (2)
  Chengwei Duan (3)
  Jiabing Shen (4)
  Cuiying Tang (5)
  Dongmei Zhang (6)
  Yuhong Ji (1)
  
  1. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Immunology, Medical College, Nantong University, Nantong, 226001, People鈥檚 Republic of China
  2. Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, People鈥檚 Republic of China
  3. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, People鈥檚 Republic of China
  4. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, People鈥檚 Republic of China
  5. Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong, 226001, Jiangsu Province, People鈥檚 Republic of China
  6. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Department of Pathogen Biology, Medical College, Nantong University, Nantong, 226001, People鈥檚 Republic of China
  
• ISSN：1573-6830

文摘

Somatostatins are peptide hormones that regulate diverse cellular processes, such as neurotransmission, cell proliferation, apoptosis, and endocrine signaling as well as inhibiting the release of many hormones and other secretory proteins. SSTR1 is a member of the superfamily of somatostatin receptors possessing seven-transmembrane segments. Aberrant expression of SSTR1 has been implicated in several human diseases, including pseudotumor cerebri, and oncogenic osteomalacia. In this study, we investigated a potential role of SSTR1 in the regulation of neuronal apoptosis in the course of intracerebral hemorrhage (ICH). A rat ICH model in the caudate putamen was established and subjected to behavioral tests. Western blot and immunohistochemistry indicated a remarkable up-regulation of SSTR1 expression surrounding the hematoma after ICH. Double-labeled immunofluorescence showed that SSTR1 was mostly co-localized with neurons, and was rarely distributed in activated astrocytes and microglia. Additionally, SSTR1 co-localized with active-caspase-3 and bcl-2 around the hematoma. The expression of active-caspase-3 was parallel with that of SSTR1 in a time-dependent manner. In addition, SSTR1 knockdown specifically resulted in reduced neuronal apoptosis in PC12 cells. All our findings suggested that up-regulated SSTR1 contributed to neuronal apoptosis after ICH, which was accompanied with reduced expression of bcl-2.