Faraday cage-type electrochemiluminescence immunosensor for ultrasensitive detection of Vibrio vulnificus based on multi-functionalized graphene oxide

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• 作者：Zhiyong Guo ; Yuhong Sha ; Yufang Hu ; Zhongqing Yu…
• 关键词：Faraday cage ; type electrochemiluminescence immunosensor ; Vibrio vulnificus ; Multi ; functionalized graphene oxide ; Outer Helmholtz plane
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：408
• 期：25
• 页码：7203-7211
• 全文大小：1,003 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650
• 卷排序：408

文摘

A novel Faraday cage-type electrochemiluminescence (ECL) immunosensor devoted to the detection of Vibrio vulnificus (VV) was fabricated. The sensing strategy was presented by a unique Faraday cage-type immunocomplex based on immunomagnetic beads (IMBs) and multi-functionalized graphene oxide (GO) labeled with (2,2′-bipyridine)(5-aminophenanthroline)ruthenium (Ru-NH₂). The multi-functionalized GO could sit on the electrode surface directly due to the large surface area, abundant functional groups, and good electronic transport property. It ensures that more Ru-NH₂ is entirely caged and become “effective,” thus improving sensitivity significantly, which resembles extending the outer Helmholtz plane (OHP) of the electrode. Under optimal conditions, the developed immunosensor achieves a limit of detection as low as 1 CFU/mL. Additionally, the proposed immunosensor with high sensitivity and selectivity can be used for the detection of real samples. The novel Faraday cage-type method has shown potential application for the diagnosis of VV and opens up a new avenue in ECL immunoassay.