Prevalence and molecular identification of Cryptosporidium spp. in pigs in Henan, China

详细信息    查看全文

• 作者：Rongjun Wang ; Shuxing Qiu ; Fuchun Jian ; Sumei Zhang ; Yujuan Shen ; Longxian Zhang ; Changshen Ning ; Jianping Cao ; Meng Qi and Lihua Xiao
• 刊名：Parasitology Research
• 出版年：2010
• 出版时间：November 2010
• 年：2010
• 卷：107
• 期：6
• 页码：1489-1494
• 全文大小：134.0 KB

文摘

The distribution and public health significance of Cryptosporidium species/genotypes in pigs differ among geographic areas and studies. To characterize the prevalence of cryptosporidiosis in pigs in Henan, China, a total of 1,350 fecal samples from 14 farms in ten prefectures in Henan Province were examined. The overall prevalence of Cryptosporidium was 8.2 % (111/1,350), with the highest infection rate (79/383 or 20.6 % ) in 1–2-month-old piglets and the lowest infection rates in 3–6-month-old pigs. Cryptosporidium-positive samples from 108 animals were analyzed by polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis of the small subunit rRNA gene, and 35 were further analyzed by DNA sequencing of the PCR products. Two Cryptosporidium species/genotype were identified, including Cryptosporidium suis (94/108) and the Cryptosporidium pig genotype II (14/108). C. suis infection was more common in younger piglets whereas the pig genotype II was relatively common in older pigs. These findings suggest that pigs are not a major source of zoonotic Cryptosporidium in the study area.