Liraglutide Promotes Cortical Neurite Outgrowth via the MEK–ERK Pathway

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• 作者：Meng Li ; Shilun Li ; Yukun Li
• 关键词：Liraglutide ; Cortical neuron ; Neurite outgrowth ; ERK ; p ; CREB
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：35
• 期：7
• 页码：987-993
• 全文大小：969 KB
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• 作者单位：Meng Li (1) (2)
  Shilun Li (2)
  Yukun Li (1) (2)
  
  1. Department of Endocrinology, The Third Hospital of Hebei Medical University, 139 Ziqiang Road, Shijiazhuang, 050051, Hebei Province, China
  2. Key Orthopaedic Biomechanics Laboratory of Hebei Province, 139 Ziqiang Road, Shijiazhuang, 050051, Hebei Province, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Neurosciences
  Animal Anatomy, Morphology and Histology
  
• 出版者：Springer Netherlands
• ISSN：1573-6830

文摘

Liraglutide is the glucagon-like peptide-1 (GLP-1) synthetic form which has been approved by the US Food and Drug Administration to be released onto the market. The metabolic benefits of incretin hormone as an anti-diabetic agent are widely recognized, but its potential extra-pancreatic effects of GLP-1 analog (liraglutide) in the central nerve system are less well known. To this purpose, we used immunofluorescence method to examine the effect of liraglutide on neurite outgrowth in primary cortical neuron culture by measuring neurite length and confirmed the promotion effect. Then, we investigated the potential mechanisms and found that liraglutide promoted neurite outgrowth in a dose-dependant manner, and this effect could be partially inhibited by MEK–ERK inhibitor U0126. Besides, liraglutide induced an increase of p-ERK/ERK expression, which could be blocked in the presence of U0126. Similarly, phosphorylated transcription factor (p-CREB) level shared the same trend with p-ERK/ERK ratio after liraglutide treatment. Collectively, our data illustrated that that liraglutide exerts neurotrophin-like activity partly via MEK–ERK pathway, which might offer a novel idea for treatment of axon-associated neurological diseases. Keywords Liraglutide Cortical neuron Neurite outgrowth ERK p-CREB