The Determination of Protein-Based Arsenic in Shrimp Tissues by Hydride Generation-Atomic Fluorescence Spectrometer

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• 作者：Xiaoying Liu ; Haiou Qiu ; Yunjie Huang ; Zhiyong Tang…
• 关键词：Protein ; based arsenic ; Protein extraction ; Shrimp ; HG ; AFS
• 刊名：Food Analytical Methods
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：9
• 期：1
• 页码：1-6
• 全文大小：318 KB
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• 作者单位：Xiaoying Liu (1)
  Haiou Qiu (1) (3)
  Yunjie Huang (1)
  Zhiyong Tang (1)
  Shaozhan Tang (1)
  Zhenli Zhu (2)
  
  1. Engineering Research Center of Nano-Geomaterials of Ministry of Education, China University of Geosciences, Wuhan, 430074, People’s Republic of China
  3. Zhejiang Research Institute, China University of Geosciences, Wuhan, People’s Republic of China
  2. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

A method combining protein extraction with hydride generation-atomic fluorescence spectrometer was developed to analyze protein-based arsenic (PBA) present in popular shrimp of China. The traditional method of alkali extraction-isoelectric precipitation was used for protein extraction, and microwave was applied for sample digestion. It was found that PBA concentrations in the extracted proteins were 0.626, 4.01, and 5.91 μg/g for meat, shell, and visceral samples, respectively. PBA recoveries of between 89.72 and 102.3 % were obtained by using the extracted proteins from meat as “standard proteins.” The results of sample and time variability tests showed that PBA concentrations in shrimp samples were very stable. The obtained total arsenic concentration in meat was only 0.123 μg/g and indicated humans’ low daily intake of arsenic from this kind of Chinese shrimp. Keywords Protein-based arsenic Protein extraction Shrimp HG-AFS