rational design and directed evolution of CYP102A1 (BM3) for regio-specific hydroxylation of isoflavone

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• 作者：Sungghi Ko ; Yung-Hun Yang ; Kwon-Young Choi…
• 关键词：cytochrome P450 monooxygenases ; self ; sufficient CYP102A1 ; isoflavone ; ortho ; specific hydroxylation ; regioselectivity
• 刊名：Biotechnology and Bioprocess Engineering
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：20
• 期：2
• 页码：225-233
• 全文大小：717 KB
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• 作者单位：Sungghi Ko (1)
  Yung-Hun Yang (2)
  Kwon-Young Choi (3) (4)
  Byung-Gee Kim (1)
  
  1. School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, Korea
  2. Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, Korea
  3. Department of Environmental Engineering, College of Engineering, Ajou University, Suwon, 443-749, Korea
  4. Graduate school of Environment and Safety Engineering, Ajou University, Suwon, 443-749, Korea
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  
• 出版者：The Korean Society for Biotechnology and Bioengineering
• ISSN：1976-3816

文摘

Isoflavonoids are a class of polycyclic aromatic hydrocarbon compounds originating from plants as secondary metabolites. Their oxidative metabolites such as ortho dihydroxyisoflavones (ODIs), where the ortho carbons of the hydroxyl groups are hydroxylated, have been reported to have a remarkably high antioxidant effect. Here, we report enzyme engineering of the cytochrome P450 enzyme CYP102A1 (BM3) for the synthesis of ODIs from isoflavonoids.