Improvement of Ansamitocin P-3 Production by Actinosynnema mirum with Fructose as the Sole Carbon Source

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• 作者：Tinglan Li ; Yuxiang Fan ; Komi Nambou ; Fengxian Hu…
• 关键词：Actinosynnema mirum ; Ansamitocin P ; 3 ; Fructose ; Glucose ; Metabolite analysis
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：175
• 期：6
• 页码：2845-2856
• 全文大小：1,810 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

Ansamitocin P-3 (AP-3) is an active and potent anti-tumor maytansinoid, which is usually produced by Actinosynnema spp. In this study, the effects of different carbon sources on biomass and AP-3 production by Actinosynnema mirum were investigated. The results showed great biomass production behavior of A. mirum in glucose medium comparatively to other carbon sources. Interestingly, when fructose was used as the sole carbon source, the highest yield of AP-3 was obtained, which was about fourfold than that of strain cultured in glucose after 168?h. Further analysis conducted in regard to better understanding of such observations in glucose and fructose defined media showed that fructose improves AP-3 production through the stimulation of the key genes of the secondary metabolism pathways. It was concluded that fructose could be a potential carbon source for cost-effective production of AP-3 from an industrial point of view.