Antiviral activity and specific modes of action of bacterial prodigiosin against Bombyx mori nucleopolyhedrovirus in vitro

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• 作者：Wei Zhou ; Cheng Zeng ; RenHua Liu ; Jie Chen ; Ru Li…
• 关键词：Prodigiosin ; Antiviral activity ; Bombyx mori nucleopolyhedrovirus ; Silkworm
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：100
• 期：9
• 页码：3979-3988
• 全文大小：823 KB
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• 作者单位：Wei Zhou (1)
  Cheng Zeng (1)
  RenHua Liu (1)
  Jie Chen (1)
  Ru Li (1)
  XinYan Wang (1)
  WenWen Bai (1)
  XiaoYuan Liu (1)
  TingTing Xiang (1)
  Lin Zhang (1) (2)
  YongJi Wan (1)
  
  1. Laboratory of Invertebrate Pathology and Applied Microbiology, College of Biotechnology, Southwest University, Chongqing, 400716, China
  2. College of Animal Science and Technology, Southwest University, Chongqing, 400716, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Prodigiosin, the tripyrrole red pigment, is a bacterial secondary metabolite with multiple bioactivities; however, the antiviral activity has not been reported yet. In the present study, we found the antiviral activity of bacterial prodigiosin on Bombyx mori nucleopolyhedrovirus (BmNPV)-infected cells in vitro, with specific modes of action. Prodigiosin at nontoxic concentrations selectively killed virus-infected cells, inhibited viral gene transcription, especially viral early gene ie-1, and prevented virus-mediated membrane fusion. Under prodigiosin treatment, both progeny virus production and viral DNA replication were significantly inhibited. Fluorescent assays showed that prodigiosin predominantly located in cytoplasm which suggested it might interact with cytoplasm factors to inhibit virus replication. In conclusion, the present study clearly indicates that prodigiosin possesses significant antiviral activity against BmNPV.