FTY720, a sphingosine-1-phosphate (S1P) receptor modulator, protects sinusoid endothelial cells from radiation injury in vitro

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• 作者：Shu-Min Zhang (1)
  Yi-Xing Chen (1)
  Jing Sun (1)
  Lei Guo (2)
  Zhao-Chong Zeng (1)
  
  1. Department of Radiation Oncology ; Zhongshan Hospital ; Fudan University ; #180 ; Fenglin Road ; Shanghai ; China
  2. Institute of Liver Cancer ; Zhongshan Hospital ; Fudan University ; Shanghai ; 200032 ; China
  
• 关键词：FTY720 ; S1P ; Sinusoid endothelial cells ; Radiation ; induced liver disease ; Akt pathway
• 刊名：Hepatology International
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：9
• 期：1
• 页码：149-154
• 全文大小：539 KB
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• 刊物主题：Hepatology; Colorectal Surgery; Surgery;
• 出版者：Springer India
• ISSN：1936-0541

文摘

Purpose To determine whether FTY720, a sphingosine-1-phosphate (S1P) receptor modulator, would protect sinusoid endothelial cells (SECs) from radiation injury in vitro. Materials and methods The effect of FTY720 on the viability of irradiated human liver SECs were examined by MTT assay or FACS analysis. The effect of FTY720 on the survival of hepatocellular carcinoma cell line, HepG2 and McA-RH7777, were determined by clonogenic assays. The activation of Akt pathway was tested by western bolt. Results FTY720 increases the survival of irradiated SECs; in contrast, it does not appear to be radioprotective of tumor cells. Furthermore, the activation of Akt pathway was confirmed in the protective effect of FTY720 on SECs. Conclusion These results suggest that FTY720 will be a potential therapeutic protector for the SEC apoptosis during RILD.