Metabolic engineering of indole pyruvic acid biosynthesis in Escherichia coli with tdiD

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• 作者：Yelin Zhu ; Yan Hua ; Biao Zhang ; Lianhong Sun ; Wenjie Li…
• 关键词：Indole pyruvic acid ; tdiD ; Aminotransferase ; tnaA
• 刊名：Microbial Cell Factories
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：16
• 期：1
• 全文大小：1466KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Applied Microbiology; Biotechnology; Microbiology; Microbial Genetics and Genomics; Enzymology; Genetic Engineering;
• 出版者：BioMed Central
• ISSN：1475-2859
• 卷排序：16

文摘

BackgroundIndole pyruvic acid (IPA) is a versatile platform intermediate and building block for a number of high-value products in the pharmaceutical and food industries. It also has a wide range of applications, such as drugs for the nervous system, cosmetics, and luminophores. Chemical synthesis of IPA is a complicated and costly process. Moreover, through the biosynthesis route employing l-amino acid oxidase, the byproduct hydrogen peroxide leads the degradation of IPA. TdiD, identified as a specific tryptophan aminotransferase, could be an alternative solution for efficient IPA biosynthesis.