KNOTTED1 mRNA undergoes long-distance transport and interacts with movement protein binding protein 2C in pear (Pyrus betulaefolia)

详细信息    查看全文

• 作者：Xuwei Duan ; Wenna Zhang ; Jing Huang…
• 关键词：Pyrus betulaefolia Bunge ; PbKN1 ; PbMPB2C ; mRNA transport ; Interaction
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：121
• 期：1
• 页码：109-119
• 全文大小：6,174 KB
• 参考文献：1. Banerjee, AK, Chatterjee, M, Yu, Y (2006) Dynamics of a mobile RNA of potato involved in a long-distance signaling pathway. Plant Cell 18: pp. 3443-3457 CrossRef
  2. Banerjee, AK, Lin, T, Hannapel, DJ (2009) Untranslated regions of a mobile transcript mediate RNA metabolism. Plant Physiol 151: pp. 1831-1843 CrossRef
  3. Box, MS, Dodsworth, S, Rudall, PJ, Bateman, RM, Glover, BJ (2012) Flower-specific KNOX phenotype in the orchid Dactylorhiza fuchsii. J Exp Bot 63: pp. 695-709 CrossRef
  4. Chang, S, Puryear, J, Cairney, J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11: pp. 113-116 CrossRef
  5. Cho, SY, Cho, WK, Choi, HS, Kim, KH (2012) Cis-acting element (SL1) of Potato virus X controls viral movement by interacting with the NbMPB2Cb and viral proteins. Virology 427: pp. 166-176 CrossRef
  6. Curin, M, Ojangu, EL, Trutnyeva, K, Ilau, B, Truve, E, Waigmann, E (2007) MPB2C, a microtubule-associated plant factor, is required for microtubular accumulation of tobacco mosaic virus movement protein in plants. Plant Physiol 143: pp. 801-811 CrossRef
  7. Deeken, R, Ache, P, Kajahn, I (2008) Identification of Arabidopsis thaliana phloem RNAs provides a search criterion for phloem-based transcripts hidden in complex datasets of microarray experiments. Plant J 55: pp. 746-759 CrossRef
  8. Felsenstein, J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: pp. 783-791 CrossRef
  9. Fichtenbauer, D, Xu, XM, Jackson, D, Kragler, F (2012) The chaperonin CCT8 facilitates spread of tobamovirus infection. Plant Signal Behav 7: pp. 318-321 CrossRef
  10. Gehring, WJ, Qian, YQ, Billeter, M, Furukubo-Tokunaga, K, Schier, AF, Resendez-Perez, D, Affolter, M, Otting, G, Wüthrich, K (1994) Homeodomain-DNA recognition. Cell 78: pp. 211-223 CrossRef
  11. Gómez, G, Torres, H, Pallas, V (2005) Identification of translocatable RNA-binding phloem proteins from melon, potential components of the long-distance RNA transport system. Plant J 41: pp. 107-116 CrossRef
  12. Ham, BK, Brandom, JL, Xoconostle-Cázares, B, Ringgold, V, Lough, TJ, Lucas, WJ (2009) A polypyrimidine tract binding protein, pumpkin RBP50, forms the basis of a phloem-mobile ribonucleoprotein complex. Plant Cell 21: pp. 197-215 CrossRef
  13. Haywood, V, Yu, TS, Huang, NC (2005) Phloem long-distance trafficking of GIBBERELLIC ACID-INSENSITIVE RNA regulates leaf development. Plant J 42: pp. 49-68 CrossRef
  14. Jones, DT, Taylor, WR, Thornton, JM (1992) The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci 8: pp. 275-282
  15. Kanehira, A, Yamada, K, Iwaya, T, Tsuwamoto, R, Kasai, A, Nakazono, M, Harada, T (2009) Apple phloem cells contain some mRNAs transported over long distances. Tree Genet Genomes 6: pp. 635-642 CrossRef
  16. Kerstetter, R, Vollbrecht, E, Lowe, B, Veit, B, Yamaguchi, J, Hake, S (1994) Sequence analysis and expression patterns divide the maize knotted1-like homeobox genes into two classes. Plant Cell 12: pp. 1877-1887 CrossRef
  17. Kim, M, Canio, W, Kessler, S (2001) Developmental changes due to long-distance
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

KNOTTED1 (KN1)-like homologous box is a homeodomain transcription factor that is transported intercellularly through plasmodesmata and over long distances via phloem transport in plants. In this study, we investigated KN1 long-distance transport and its regulation by the viral movement protein binding protein of ‘Du Li-(Pyrus betulaefolia, PbMPB2C) in two species of fruit trees, ‘Du Li-(rootstock) and ‘Ya Li-(P. bretschneideri, scion), using grafting experiments. The results showed that PbKN1 mRNA was present in the phloem in both transport directions across the graft junctions of tissue-cultured grafted plants and orchard saplings. To investigate the relationship between PbKN1 and PbMPB2C, we conducted interaction assays to demonstrate their physical interaction. An RNA–protein pull-down assay revealed that PbKN1 mRNA interacted with PbMPB2C protein in vitro but not with PbKN1 and that PbKN1 repressed the binding of PbMPB2C to PbKN1 mRNA. Additionally, using p35S:GFP-PbKN1 transgenic tobacco grafted with wild-type plants, we found that the movement of PbKN1 was increased by the silencing of NtMPB2C. Based on the above findings, we first demonstrate evidence of the bidirectional transport of PbKN1 mRNA and propose that PbMPB2C could act as a negative regulator of the phloem-mediated long-distance transport of PbKN1 mRNA.