Immunogenicity of transmissible gastroenteritis virus (TGEV) M gene delivered by attenuated Salmonella typhimurium in mice

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• 作者：Ying Qing ; Jiawen Liu ; Xiaobo Huang ; Yaqing Li ; Yudi Zhang ; Jie Chen…
• 关键词：Transmissible gastroenteritis virus ; Attenuated Salmonella typhimurium ; M gene
• 刊名：Virus Genes
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：52
• 期：2
• 页码：218-227
• 全文大小：1,240 KB
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• 作者单位：Ying Qing (1)
  Jiawen Liu (1) (2)
  Xiaobo Huang (1)
  Yaqing Li (1)
  Yudi Zhang (1)
  Jie Chen (1)
  Xintian Wen (1)
  Sanjie cao (1)
  Yiping Wen (1)
  Rui Wu (1)
  Qigui Yan (1)
  Xiaoping Ma (1)
  
  1. Research Center of Swine Disease, Infectious Disease and Microarray, College of Veterinary Medicine, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu, 611130, China
  2. Sichuan Key Laboratory of Conservation Biology for Endangered Wild Life, Chengdu Research Base of Giant Panda Breeding, Chengdu, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Virology
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-994X

文摘

Attenuated Salmonella typhimurium (S. typhimurium) was selected as a transgenic vehicle for the development of live mucosal vaccines against transmissible gastroenteritis virus (TGEV) based on the M gene. An approximate 1.0 kb DNA fragment, encoding for glycoprotein M, was amplified by RT-PCR and cloned into eukaryotic expression vector pVAX1. The recombinant plasmid pVAX-M was transformed by electroporation into attenuated S. typhimurium SL7207, and the expression and translation of the pVAX-M delivered by recombinant S. typhimurium SL7207 (pVAX-M) was detected both in vitro and in vivo. BALB/c mice were inoculated orally with SL7207 (pVAX-M) at different dosages to evaluate safety of the vaccines. The bacterium was safe to mice at a dosage of 2 × 109 CFU, almost eliminated from the spleen and liver at week 4 post-immunization and eventually cleared at week 6. Mice immunized with 1 × 109 CFU of SL7207 (pVAX-M) elicited specific anti-TGEV local mucosal and humoral responses including levels of IgA, IgG, IL-4, and IFN-γ as measured by indirect ELISA assay. Moreover, the control groups (pVAX group, PBS group) maintained at a normal level during week 4–8 post-immunization. The results indicated that attenuated S. typhimurium could be used as a delivery vector for oral immunization of TGEV M gene vaccine.