Background and aimsZinc (Zn) nutrition affects wheat yield and the health of humans who consume wheat grain. This study determined: 1) how distributions of roots and available Zn (DTPA-Zn) in soil affect the Zn content of wheat plants; and 2) the concentrations of shoot Zn and soil DTPA-Zn needed to obtain high yields and the “target value” of grain Zn biofortification (45 mg kg−1).