The effect of pregnancy on paternal skin allograft survival

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• 作者：ZhangFei Shou (1) (2) (3)
  YiFang Xu (2)
  HuaYing Xiao (2)
  Qin Zhou (2)
  JieRu Cai (2)
  Yi Yang (1)
  Hong Jiang (2)
  WenJie Zhang (3)
  JiangHua Chen (1) (2)
  
• 关键词：regulatory T cells ; pregnancy ; maternal ; fetal tolerance ; transplant tolerance ; survival ; MLR ; paternal skin allograft ; mice
• 刊名：Science China Life Sciences
• 出版年：2009
• 出版时间：November 2009
• 年：2009
• 卷：52
• 期：11
• 页码：1011-1015
• 全文大小：1429KB
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• 作者单位：ZhangFei Shou (1) (2) (3)
  YiFang Xu (2)
  HuaYing Xiao (2)
  Qin Zhou (2)
  JieRu Cai (2)
  Yi Yang (1)
  Hong Jiang (2)
  WenJie Zhang (3)
  JiangHua Chen (1) (2)
  
  1. Kidney Disease Center, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China
  2. Key Laboratory of Multi-organ Combined Transplantation, Ministry of Health, Hangzhou, 310003, China
  3. Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Ministry of Education, School of Medicine, Shihezi University, Shihezi, 832003, China
  

文摘

Elucidation of maternal-fetal tolerance mechanisms clarifies the role of regulatory T cells (Treg) in transplant tolerance. This study aim to investigate the effect of pregnancy on paternal skin allograft survival. Flow cytometry techniques, mixed lymphocytes reaction (MLR), PCR, real-time PCR and skin transplantation were key methods. Treg increased significantly from 4.2% before pregnancy to peak at 6.8% day 8 after pregnancy. Both heme oxygenase-1 (HO-1) and indoleamine 2,3-dioxygenase (IDO) mRNA express high in placenta while low in spleen (P<0.05). Although Treg increased during pregnancy, and splenocytes from the pregnant mice showed lower MLR response toward the paternal stimulator, single time pregnancy showed no significant protective effect on paternal skin allograft survival in the tested condition.