Comprehensive analysis of expressed sequence tags from cultivated and wild radish (Raphanus spp.)
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- 作者：Di Shen (8) (9)
Honghe Sun (10) (9)
Mingyun Huang (9)
Yi Zheng (9)
Yang Qiu (8)
Xixiang Li (8)
Zhangjun Fei (11) (9) - 关键词：Radish ; EST ; SNP ; SSR ; Comparative analysis ; Whole genome duplication ; Phylogenetic relationship
- 刊名：BMC Genomics
- 出版年：2013
- 出版时间：December 2013
- 年：2013
- 卷：14
- 期：1
- 全文大小：535 KB
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Honghe Sun (10) (9)
Mingyun Huang (9)
Yi Zheng (9)
Yang Qiu (8)
Xixiang Li (8)
Zhangjun Fei (11) (9)
8. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
9. Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, 14853, USA
10. National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China
11. U.S. Department of Agriculture/Agriculture Research Service, Robert W. Holley Centre for Agriculture and Health, Ithaca, NY, 14853, USA - ISSN：1471-2164
文摘
Background Radish (Raphanus sativus L., 2n--×--8) is an economically important vegetable crop worldwide. A large collection of radish expressed sequence tags (ESTs) has been generated but remains largely uncharacterized. Results In this study, approximately 315,000 ESTs derived from 22 Raphanus cDNA libraries from 18 different genotypes were analyzed, for the purpose of gene and marker discovery and to evaluate large-scale genome duplication and phylogenetic relationships among Raphanus spp. The ESTs were assembled into 85,083 unigenes, of which 90%, 65%, 89% and 89% had homologous sequences in the GenBank nr, SwissProt, TrEMBL and Arabidopsis protein databases, respectively. A total of 66,194 (78%) could be assigned at least one gene ontology (GO) term. Comparative analysis identified 5,595 gene families unique to radish that were significantly enriched with genes related to small molecule metabolism, as well as 12,899 specific to the Brassicaceae that were enriched with genes related to seed oil body biogenesis and responses to phytohormones. The analysis further indicated that the divergence of radish and Brassica rapa occurred approximately 8.9-14.9 million years ago (MYA), following a whole-genome duplication event (12.8-21.4 MYA) in their common ancestor. An additional whole-genome duplication event in radish occurred at 5.1-8.4 MYA, after its divergence from B. rapa. A total of 13,570 simple sequence repeats (SSRs) and 28,758 high-quality single nucleotide polymorphisms (SNPs) were also identified. Using a subset of SNPs, the phylogenetic relationships of eight different accessions of Raphanus was inferred. Conclusion Comprehensive analysis of radish ESTs provided new insights into radish genome evolution and the phylogenetic relationships of different radish accessions. Moreover, the radish EST sequences and the associated SSR and SNP markers described in this study represent a valuable resource for radish functional genomics studies and breeding.