Pluripotent-related gene expression analyses in single porcine recloned embryo

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• 作者：Yongye Huang (1)
  Wanhua Xie (1)
  Chaogang Yao (1)
  Yang Han (1)
  Guangyun Tan (1)
  Yang Zhou (1)
  Jianguo Zhu (1)
  Daxin Pang (1)
  Zhanjun Li (1)
  Xiaochun Tang (1)
  
• 关键词：Blastocyst formation ; Embryo development ; Porcine embryos ; Single ; embryo mRNA expression ; Somatic cell nuclear transfer ; Valproic acid
• 刊名：Biotechnology Letters
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：36
• 期：6
• 页码：1161-1169
• 全文大小：
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• 作者单位：Yongye Huang (1)
  Wanhua Xie (1)
  Chaogang Yao (1)
  Yang Han (1)
  Guangyun Tan (1)
  Yang Zhou (1)
  Jianguo Zhu (1)
  Daxin Pang (1)
  Zhanjun Li (1)
  Xiaochun Tang (1)
  
  1. Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, 130062, Jilin, China
  
• ISSN：1573-6776

文摘

The developmental ability among embryos produced by three different techniques were examined: there were no significant differences in the developmental rate in porcine embryos produced by in vitro fertilization (IVF) and first generation of somatic cell nucleus transfer (SCNT), but the developmental rate dropped sharply at the 2- to four-cell stage in recloned (second generation of SCNT) embryos. In most recloned embryos, Oct4 and Klf4 were under-expressed at all stages, whereas Sox2 and Nanog were over-expressed at the two-cell stage. In contrast, Nanog was absent in IVF and SCNT embryos at the two-cell stage. The recloned embryos were treated with valproic acid to enhance developmental capacity and this led to an increase in the rate of blastocyst formation and total cell number compared with the findings for untreated recloned embryos (29.8 vs. 12.4?%, 39 vs. 25, respectively, p?<?0.05).