Transcriptomic analysis of aflatoxin B1-regulated genes in rat hepatic epithelial cells

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• 作者：Liu Yang (1)
  Jing Ji (2)
  Guanghui Li (3)
  Junwen Li (4)
  Zhaoli Chen (4)
  Haiyong Wang (2)
  
• 关键词：aflatoxin ; microarray ; gene ontology ; Kyoto encyclopedia of genes and genomes(KEGG) pathway ; hepatotoxicity
• 刊名：Transactions of Tianjin University
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：20
• 期：6
• 页码：451-457
• 全文大小：663 KB
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• 作者单位：Liu Yang (1)
  Jing Ji (2)
  Guanghui Li (3)
  Junwen Li (4)
  Zhaoli Chen (4)
  Haiyong Wang (2)
  
  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China
  2. School of Environmental Science and Technology, Tianjin University, Tianjin, 300072, China
  3. Department of Chemistry, Tangshan Normal University, Tangshan, 063000, China
  4. Institute of Hygiene and Environmental Medicine, Academy of Military Medical Science, Tianjin, 300050, China
  
• ISSN：1995-8196

文摘

Aflatoxins are the most popular hepatotoxicants. Chronic exposure to aflatoxins leads to a wide variety of liver diseases, such as hepatocellular carcinoma. In this study, we analyzed the genome wide expression profiles of aflatoxin B1-induced rat hepatic epithelial cells. The expression of 325, 184 and 199 special genes was altered when exposed to 0.03, 0.1 and 0.2 μmol/L aflatoxin B1 respectively, and 239 genes were commonly expressed. After the functional analysis on these dose-special genes, we determined several key pathways related to hepatotoxicity, such as TGF-beta signaling pathway, tight junction, adherens junction, the regulation of actin cytoskeleton, ErbB signaling pathway, p53 signaling pathway, pathways in cancer and axon guidance. Common genes were mainly associated with focal adhesion, ECM-receptor interaction, and cell adhesion molecules. Gene ontology annotations showed a good concordance with these pathways. The quantitative real-time polymerase chain reaction(PCR) analysis of selected genes showed similar patterns in microarrays. The toxicogenomic study provides a better understanding of molecular mechanisms of aflatoxins.