Characterization of eleven microsatellite markers for the tea geometrid Ectropis obliqua (Lepidoptera: Geometridae)

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• 作者：Shengli Jing ; Baoxiao Zheng ; Li Qiao ; Kun Zhang…
• 关键词：Genetic diversity ; Genomic library ; Geometridae ; Lepidoptera ; Microsatellite
• 刊名：Applied Entomology and Zoology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：51
• 期：4
• 页码：673-676
• 全文大小：412 KB
• 刊物主题：Entomology; Agriculture; Applied Ecology; Plant Pathology; Zoology; Environmental Management;
• 出版者：Springer Japan
• ISSN：1347-605X
• 卷排序：51

文摘

The tea geometrid, Ectropis obliqua Prout (Lepidoptera: Geometridae), is one of the most threatening pests of tea (Camellia sinensis (L.) O. Kuntze) plants. In this study, we developed eleven new polymorphic microsatellite markers from tea geometrid by using the microsatellite motif enriched library method. Polymorphism of each locus was detected in 69 individuals from three natural populations. The number of alleles varied from 2 to 8, and the expected and observed heterozygosities ranged from 0.042 to 0.801 and from 0.042 to 0.875, respectively. Six loci had significant deviations from the Hardy–Weinberg equilibrium (HWE). These microsatellite markers will be useful for the future study of this agricultural pest in genetic diversity, population structure and evolution of resistance. This information will be allowed to establish new pest management strategies and improve the effectiveness of pest control program of this species.