AFLP analysis of genetic diversity and relationship among some Chinese domestic ducks and wild ducks

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• 作者：Feihuan Yan (1)
  Zhenghong Zuo (1)
  Mei Chen (1)
  Yueqiang Song (1)
  Liangju Lü (1)
  Yixin Chen (1)
  
• 关键词：amplified fragment length polymorphic (AFLP) ; domestic duck ; genetic diversity ; wild duck
• 刊名：Frontiers in Biology
• 出版年：2006
• 出版时间：September 2006
• 年：2006
• 卷：1
• 期：3
• 页码：280-284
• 全文大小：622KB
• 参考文献：1. Chen Y. X., Zhao Y., Lu L. J., Lai Y. Z., Comparative study of mitochondrial DNA restriction maps of genus anas ducks in Fujian Province. Journal of Xiamen University (Natural Science), 1999, 38(1): 108-11 [ , DNA (mtDNA) . (, 1999, 38(1): 108-11]
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• 作者单位：Feihuan Yan (1)
  Zhenghong Zuo (1)
  Mei Chen (1)
  Yueqiang Song (1)
  Liangju Lü (1)
  Yixin Chen (1)
  
  1. School of Life Science, Xiamen University, Xiamen, 361005, China
  
• ISSN：1674-7992

文摘

The amplified fragment length polymorphic (AFLP) technique was used to analyze the genome DNA polymorphism among 8 breeds of domestic ducks and 2 species of wild ducks. Nine of the 17 selected primers pairs gave reproducible polymorphic DNA amplification bands. The amplified bands ranged from 44 to 83 per primer pair. Of the 513 AFLP markers obtained, 498 were polymorphic. The proportion of polymorphic loci was 97.1%. The genetic distance (D) and similarity coefficients (GS) were calculated based on the polymorphic data. Between domestic ducks D ranged from 0.331 to 0.589, while between domestic ducks and the wild ducks, it ranged from 0.298 to 0.520 (vs. Anas Platyrhynchos) and from 0.316 to 0.522 (vs. A. Poecilorhyncha), respectively. The variance analysis showed no significant difference between the two groups of data, which indicated that both mallard and spot-billed ducks made contributions to domestic duck evolution. A dendrogram was constructed according to the D value.