Effects of Paclobutrazol Exposure on Antioxidant Defense System in Sebastiscus marmoratus

详细信息    查看全文

• 作者：Jinshou Li (1)
  Lingbin Sun (2)
  Zhenghong Zuo (2)
  Meng Chen (3)
  Chonggang Wang (2)
  
• 关键词：Pesticide ; Paclobutrazol ; Antioxidant defense system ; Marine fish
• 刊名：Bulletin of Environmental Contamination and Toxicology
• 出版年：2012
• 出版时间：October 2012
• 年：2012
• 卷：89
• 期：4
• 页码：723-726
• 全文大小：255KB
• 参考文献：1. Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133-40
  2. Bradford MMA (1976) Rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Chem 72:248-54
  3. Castillo LE, Martinez E, Ruepert C, Savage C, Gilek M, Pinnock M, Solis E (2006) Water quality and macroinvertebrate community response following pesticide applications in a banana plantation, Limon, Costa Rica. Sci Total Environ 367:418-32 CrossRef
  4. Ding F, Song WH, Guo J, Gao ML, Hu WX (2009) Oxidative stress and structure-activity relationship in the zebrafish ( / Danio rerio) under exposure to paclobutrazol. J Environ Sci Health B 44:44-0 CrossRef
  5. Egaas E, Sandvik M, Fjeld E, Kallqvist T, Goks?yr A, Svensen A (1999) Some effects of the fungicide propiconazole on cytochrome P450 and glutathione S-transferase in brown trout ( / Salmo trutta). Comp Biochem Physiol C 122:337-44 CrossRef
  6. Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130-139
  7. Hafeman DG, Sunde RA, Hoekstra WG (1973) Effect of dietary selenium and erythrocyte and liver glutathione peroxidase in the rat. J Nutr 104:580
  8. Hissin PJ, Hilf RA (1976) Fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Chem 74:214-26
  9. Konwick BJ, Garrison AW, Avants JK, Fisk AT (2006) Bioaccumulation and biotransformation of chiral triazole fungicides in rainbow trout ( / Oncorhynchus mykiss). Aquat Toxicol 80:372-81 CrossRef
  10. Li ZH, Zlabek V, Grabic R, Li P, Randak T (2010a) Modulation of glutathione-related antioxidant defense system of fish chronically treated by the fungicide propiconazole. Comp Biochem Physiol C 152:392-98
  11. Li ZH, Zlabek V, Li P, Grabic R, Velisek J, Machova J, Randak T (2010b) Biochemical and physiological responses in liver and muscle of rainbow trout after long-term exposure to propiconazole. Ecotoxicol Environ Saf 73:1391-396 CrossRef
  12. Li ZH, Zlabek V, Grabic R, Li P, Machova J, Velisek J, Randak T (2010c) Effects of exposure to sublethal propiconazole on the antioxidant defense system and Na⁺–K⁺–ATPase activity in brain of rainbow trout, / Oncorhynchus mykiss. Aquat Toxicol 98:297-03 CrossRef
  13. Li JS, Sun LB, Zuo ZH, Chen M, Geng H, Wang CG (2012) Exposure to paclobutrazol disrupts spermatogenesis in male / Sebastiscus marmoratus. Aquat Toxicol 122-23:120-24 CrossRef
  14. Livingstone DR (1988) Responses of microsomal NADPH-cytochrome c reductase activity and cytochrom P-450 in digestive glands of Mytilus edulis and Littorea to enviromental and experimental exposure to pollutants. Mar Ecol Prog Ser 46:37-3 CrossRef
  15. Pandey S, Parvez S, Ansari RA, Ali M, Kaur M, Hayat F, Ahmad F, Raisuddin S (2008) Effects of exposure to multiple trace metals on biochemical, histologicaland ultrastructural features of gills of a freshwater fish, Channa punctata Bloch. Chem Biol Interact 174:183-92 CrossRef
  16. Van De Steene JC, Lambert WE (2008) Validation of a solid-phase extraction and liquid chromatography—electrospray tandem mass spectrometric method for the determination of nine basic pharmaceuticals in wastewater and surface water samples. J Chromatogr A 1182:153-60 CrossRef
  17. Van der Oost R, Beyer J, Vermeulen NPE (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ Toxicol Pharmacol 13:57-49 CrossRef
  
• 作者单位：Jinshou Li (1)
  Lingbin Sun (2)
  Zhenghong Zuo (2)
  Meng Chen (3)
  Chonggang Wang (2)
  
  1. Department of Bioengineering, Ningde Normal University, Ningde, Fujian, China
  2. School of Life Sciences, Xiamen University, Xiamen, Fujian, China
  3. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China
  
• ISSN：1432-0800

文摘

This study was conducted to detect the effects of paclobutrazol (PBZ) at environmental concentration on the antioxidant defense system in Sebastiscus marmoratus. Fish were exposed to concentrations of PBZ (10, 100, 1,000?ng/L) for 50?days. The results showed: (1) The glutathione contents in the liver and brain were significantly decreased in a dose-dependent manner. (2) The activities of glutathione S-transferases (GST) and catalase in liver and brain were inhibited as the increase of PBZ concentration. A highest 2.16-fold (p?=?0.05) and 11.54-fold (p?<?0.001) reduction of GST activity in the liver and brain respectively was observed in 1,000?μg/L group. (3) The activities of glutathione peroxidase in liver were inhibited. These results suggest that the exposure of PBZ would influence the antioxidant ability of S. marmoratus.