Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response

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• 作者：Ke Huang (1)
  PengFei Liu (1) (2)
  Xiang Li (1)
  ShuBin Chen (1)
  LiHui Wang (1)
  Li Qin (3)
  ZhengHui Su (1)
  WenHao Huang (1)
  JuLi Liu (1)
  Bei Jia (4)
  Jie Liu (4)
  JingLei Cai (1)
  DuanQing Pei (1)
  GuangJin Pan (1)
  
• 关键词：induced pluripotent stem cells ; immunogenicity ; iPSC ; derived neural progenitor cells
• 刊名：Science China Life Sciences
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：57
• 期：2
• 页码：162-170
• 全文大小：939 KB
• 作者单位：Ke Huang (1)
  PengFei Liu (1) (2)
  Xiang Li (1)
  ShuBin Chen (1)
  LiHui Wang (1)
  Li Qin (3)
  ZhengHui Su (1)
  WenHao Huang (1)
  JuLi Liu (1)
  Bei Jia (4)
  Jie Liu (4)
  JingLei Cai (1)
  DuanQing Pei (1)
  GuangJin Pan (1)
  
  1. Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China
  2. Department of Regeneration Medicine, School of Pharmaceutical Science, Jilin University, Changchun, 130021, China
  3. Laboratory of Pathogen Biology, State Key Laboratory of Respiratory Disease, Center for Infection and Immunity, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China
  4. Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China
  
• ISSN：1869-1889

文摘

The breakthrough development of induced pluripotent stem cells (iPSCs) raises the prospect of patient-specific treatment for many diseases through the replacement of affected cells. However, whether iPSC-derived functional cell lineages generate a deleterious immune response upon auto-transplantation remains unclear. In this study, we differentiated five human iPSC lines from skin fibroblasts and urine cells into neural progenitor cells (NPCs) and analyzed their immunogenicity. Through co-culture with autogenous peripheral blood mononuclear cells (PBMCs), we showed that both somatic cells and iPSC-derived NPCs do not stimulate significant autogenous PBMC proliferation. However, a significant immune reaction was detected when these cells were co-cultured with allogenous PBMCs. Furthermore, no significant expression of perforin or granzyme B was detected following stimulation of autogenous immune effector cells (CD3+CD8?/sup> T cells, CD3+CD8+ T cells or CD3?/sup>CD56+ NK cells) by NPCs in both PBMC and T cell co-culture systems. These results suggest that human iPSC-derived NPCs may not initiate an immune response in autogenous transplants, and thus set a base for further preclinical evaluation of human iPSCs.