文摘
Histidine triad nucleotide binding protein 1(HINT1) plays an important role in many biological processes especially in cell biology, and they have been found in a wide variety of species. However, the functional attributes of HINT1 homologues in plants have not yet been reported. We have previously isolated the full-length cDNA of an HINT1 homologue from maize (Zea mays L.), designated as Zm-HINT1. Subcellular localization was analysed after particle-mediated transient transformation of onion epidermal cells. The result revealed that Zm-HINT1 was targeted to the nucleus. Based on real-time PCR analysis, the expression level of Zm-HINT1 increased significantly after salicylic acid or jasmonic acid treatments. To further investigate the potential physiological role of Zm-HINT1, a binary vector was constructed for Zm-HINT1 synthesis and transgenic Arabidopsis lines were generated by Agrobacterium-mediated transformation. Transgenic Zm-HINT1 plants showed increased resistance to Fusarium graminearum compared to the wild type and control plants. Futhermore, real-time PCR results revealed that the pathogen defense genes pathogenesis-related 4 (PR-4) and a peroxidase were upregulated by Zm-HINT1 overexpression. Thus, our data demonstrated that Zm-HINT1 induced antifungal activity against Fusarium graminearum and it may be useful for crop breeding in improving biotic stress tolerance.