文摘
Photochemical responses in the thylakoid membrane of Nymphoides peltatum to increasing lead (Pb) concentrations were investigated after 5?days of exposure. Pb accumulation increased in a concentration-dependent manner, with a maximum of 118.44?μg?g? at 100?μM. Nutrients (Zn, Mg, Mo, Ca, Fe and Mn), ATPase activity and pigment generally increased progressively at Pb concentrations of 12.5 and 25?μM, but then declined at concentrations of 50 and 100?μM. Moreover, Pb stress induced an increase in chlorophyll (Chl) a/b ratio in a different extent. No outstanding changes were observed in several Chl a fluorescence parameters at low Pb concentrations (12.5 and 25?μM), while significant changes (p?ATPase activity and pigment content were associated with disturbances in the thylakoid membrane, indicated by the quenching of Chl a fluorescence. These results were indicative of a disarray in photochemical activities exerted by Pb phytotoxicity.