BcMctA, a putative monocarboxylate transporter, is required for pathogenicity in Botrytis cinerea

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• 作者：Zhifeng Cui ; Nana Gao ; Qian Wang ; Yun Ren ; Kun Wang ; Tingheng Zhu
• 关键词：Botrytis cinerea ; Monocarboxylate transporter ; Gene disruption ; Functional analysis
• 刊名：Current Genetics
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：61
• 期：4
• 页码：545-553
• 全文大小：3,970 KB
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• 作者单位：Zhifeng Cui (1)
  Nana Gao (1)
  Qian Wang (1)
  Yun Ren (1)
  Kun Wang (1)
  Tingheng Zhu (1)
  
  1. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, People鈥檚 Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbial Genetics and Genomics
  Microbiology
  Biochemistry
  Cell Biology
  Plant Sciences
  Proteomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0983

文摘

Monocarboxylate transporters have a central role in mammalian metabolism, but rarely reported in phytopathogenic fungi. In this study, a putative monocarboxylate transporter gene in Botrytis cinerea [B. cinerea MctA (BcMctA)] was identified in the research of a B. cinerea transfer DNA (T-DNA) insertional mutant (74). Disruption of the gene decreased the growth rate on the medium with monocarboxylate (acetate or pyruvate) as the sole carbon sources, but not affected on lactate. The pyruvate contents in BcmctA deletion mutants decreased about 35 % compared with the wild strain. Besides, the conidial yield was increased about two times in BcmctA disruption mutant. The pathogenicity assay indicated that disruption of BcmctA significantly reduced the virulence of B. cinerea on cucumber and tomato leaves. Our results demonstrated that BcMctA is related to pyruvate uptake and pathogenicity of B. cinerea on cucumber and tomato leaves. Keywords Botrytis cinerea Monocarboxylate transporter Gene disruption Functional analysis