Screening and identification of a microsatellite marker associated with sex in Wami tilapia, Oreochromis urolepis hornorum

详细信息    查看全文

• 作者：HUAPING ZHU ; ZHIGANG LIU ; MAIXIN LU ; FENGYING GAO ; XIAOLI KE…
• 刊名：Journal of Genetics
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：95
• 期：2
• 页码：283-289
• 全文大小：2,992 KB
• 刊物主题：Life Sciences, general; Microbial Genetics and Genomics; Plant Genetics & Genomics; Animal Genetics and Genomics; Evolutionary Biology;
• 出版者：Springer India
• ISSN：0973-7731
• 卷排序：95

文摘

In this study, primer pairs of 15 microsatellite markers associated with sex determination of tilapia were selected and amplified in Wami tilapia, Oreochromis urolepis hornorum. While one marker, UNH168, on linkage group 3 (LG3) was associated (P < 0.001) with the phenotypic sex in the experimental population, nine genotypes were detected in both sexes. Only 99-bp allele was detected in the female samples, while 141, 149 and 157-bp alleles were present in both male and female samples. UNH168 was localized by fluorescence in situ hybridization (FISH) on the long arm of the largest tilapia chromosome pair (chromosome 1, equivalent to LG3). This sex-linked microsatellite marker could potentially be used for marker-assisted selection in tilapia breeding programmes to produce monosex male tilapia.Keywordsmicrosatellitesex-linked markerchromosome localizationfluorescence in situ hybridizationOreochromis urolepis hornorum.