Dual silencing of DmCPD and DmGA20ox genes generates a novel miniature and delayed-flowering Dendranthema morifolium variety
详细信息 查看全文
- 作者：Qiaoli Xie (1)
Guoping Chen (1)
Qin Liu (1)
Zhiguo Zhu (1)
Zongli Hu (1) - 关键词：DmCPD ; DmGA20ox ; Miniaturization ; Chrysanthemum ; Hormone ; Flowering
- 刊名：Molecular Breeding
- 出版年：2015
- 出版时间：February 2015
- 年：2015
- 卷：35
- 期：2
- 全文大小：1,162 KB
- 参考文献：1. Abel S, Nguyen MD, Theologis A (1995) The PS-IAA4/5-like family of early auxin-inducible mRNAs in / Arabidopsis thaliana. J Mol Biol 251(4):533鈥?49 CrossRef
2. Achard P, Herr A, Baulcombe DC, Harberd NP (2004) Modulation of floral development by a gibberellin-regulated microRNA. Sci Signal 131(14):3357
3. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in / Beta vulgaris. Plant Physiol 24(1):1 CrossRef
4. Azpiroz R, Wu Y, LoCascio JC, Feldmann KA (1998) An Arabidopsis brassinosteroid-dependent mutant is blocked in cell elongation. Plant Cell Online 10(2):219鈥?30 CrossRef
5. Bai M-Y, Shang J-X, Oh E, Fan M, Bai Y, Zentella R, Sun T-P, Wang Z-Y (2012) Brassinosteroid, gibberellin and phytochrome impinge on a common transcription module in Arabidopsis. Nat Cell Biol 14(8):810鈥?17 CrossRef
6. Bl谩zquez MA, Green R, Nilsson O, Sussman MR, Weigel D (1998) Gibberellins promote flowering of Arabidopsis by activating the LEAFY promoter. Plant Cell Online 10(5):791鈥?00 CrossRef
7. Bowman JL, Alvarez J, Weigel D, Meyerowitz EM, Smyth DR (1993) Control of flower development in / Arabidopsis thaliana by APETALA1 and interacting genes. Development 119(3):721鈥?43
8. Cai P, Long H, Deng G, Pan Z, Peng Z, Yu M (2012) Molecular cloning, characterization, and expression analysis of genes encoding gibberellin 20-oxidase in / Dasypyrum villosum dwarf mutant. Plant Mol Biol Report 30(5):1110鈥?116 CrossRef
9. Campbell P, Braam J (1998) Co- and/or post-translational modifications are critical for TCH4 XET activity. Plant J 15(4):553鈥?61 CrossRef
10. Chai Y-m, Zhang Q, Tian L, Li C-L, Xing Y, Qin L, Shen Y-Y (2013) Brassinosteroid is involved in strawberry fruit ripening. Plant Growth Regul 69(1):63鈥?9 CrossRef
11. Chen G, Hackett R, Walker D, Taylor A, Lin Z, Grierson D (2004) Identification of a specific isoform of tomato lipoxygenase (TomloxC) involved in the generation of fatty acid-derived flavor compounds. Plant Physiol 136(1):2641鈥?651 CrossRef
12. Christodoulou A, Weaver R, Pool R (1968) Relation of gibberellin treatment to fruit-set, berry development, and cluster compactness in / Vitis vinifera grapes. Proc Am Soc Hortic Sci 92:301鈥?10
13. Clouse SD (2011) Brassinosteroid signal transduction: from receptor kinase activation to transcriptional networks regulating plant development. Plant Cell Online 23(4):1219鈥?230 CrossRef
14. Cockshull K, Kofranek A (1994) High night temperatures delay flowering, produce abnormal flowers and retard stem growth of cut-flower chrysanthemums. Sci Hortic 56(3):217鈥?34 CrossRef
15. Cosgrove DJ (1998) Cell wall loosening by expansins. Plant Physiol 118(2):333鈥?39 CrossRef
16. Cosgrove DJ (2000) Loosening of plant cell walls by expansins. Nature 407(6802):321鈥?26 CrossRef
17. Damann MP, Lyons RE (1996) Natural chilling and limited inductive photoperiod affect flowering in two Asteraceae genera. J Am Soc Hortic Sci 121(4):694鈥?98
18. De Grauwe L, Vandenbussche F, Tietz O, Palme K, Van Der Straeten D (2005) Auxin, ethylene and brassinosteroids: tripartite control of growth in the Arabidopsis hypocotyl. Plant Cell Physiol 46(6):827鈥?36 CrossRef
19. Depuydt S, Hardtke CS (2011) Hormone signalling crosstalk in plant growth regulation. Curr Biol 21(9):R365鈥揜373 CrossRef
20. Diener AC, Li H, Zhou W-X, Whoriskey WJ, Nes WD, Fink GR (2000) Sterol methyltransferase 1 controls the level of cholesterol in plants. Plant Cell Online 12(6):853鈥?70 CrossRef
21. Domagalska MA, Schomburg FM, Amasino RM, Vierstra RD, Nagy F, Davis SJ (2007) Attenuation of brassinosteroid signaling enhances FLC expression and delays flowering. Development 134(15):2841鈥?850 CrossRef
22. Evans LT (1998) Feeding the ten billion: plants and population growth. Cambridge University Press, Cambridge
23. Finnegan EJ, Peacock WJ, Dennis ES (1996) Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development. Proc Natl Acad Sci USA 93(16):8449鈥?454 CrossRef
24. Fu X, Harberd NP (2003) Auxin promotes Arabidopsis root growth by modulating gibberellin response. Nature 421(6924):740鈥?43 CrossRef
25. Fujioka S, Yokota T (2003) Biosynthesis and metabolism of brassinosteroids. Annu Rev Plant Biol 54(1):137鈥?64 CrossRef
26. Fujioka S, Li J, Choi Y-H, Seto H, Takatsuto S, Noguchi T, Watanabe T, Kuriyama H, Yokota T, Chory J (1997) The Arabidopsis deetiolated2 mutant is blocked early in brassinosteroid biosynthesis. Plant Cell Online 9(11):1951鈥?962 CrossRef
27. Gertsson U, Andersson E (1985) Propagation of / Chrysanthemum 脳 / hortorum and / Philodendron scandens by tissue culture. Rapport-Sveriges Lantbruksuniversitet, Institutionen foer Traedgaardsvetenskap
28. Groot SP, Bruinsma J, Karssen CM (1987) The role of endogenous gibberellin in seed and fruit development of tomato: studies with a gibberellin-deficient mutant. Physiol Plant 71(2):184鈥?90 CrossRef
29. Hedden P (2003) The genes of the green revolution. Trends Genet 19(1):5鈥? CrossRef
30. Hedden P, Hoad GV (1994) Growth regulators and crop productivity. Marcel Dekker, New York
31. Hill G (1968) Shoot formation in tissue cultures of chrysanthemum 鈥楤ronze Pride鈥? Physiol Plant 21(2):386鈥?89 CrossRef
32. Honma T, Goto K (2001) Complexes of MADS-box proteins are sufficient to convert leaves into floral organs. Nature 409(6819):525鈥?29 CrossRef
33. Iliev EA, Xu W, Polisensky DH, Oh M-H, Torisky RS, Clouse SD, Braam J (2002) Transcriptional and posttranscriptional regulation of Arabidopsis TCH4 expression by diverse stimuli. Roles of cis regions and brassinosteroids. Plant Physiol 130(2):770鈥?83 CrossRef
34. Karlsson MG, Hanscom JT (1992) Influence of low temperature on floral development in chrysanthemum. HortScience 27(6):649
35. Kaul V, Miller RM, Hutchinson JF, Richards D (1990) Shoot regeneration from stem and leaf explants of / Dendranthema grandiflora Tzvelev (syn. / Chrysanthemum morifolium Ramat.). Plant Cell Tissue Organ Cult 21(1):21鈥?0 CrossRef
36. Kauschmann A, Jessop A, Koncz C, Szekeres M, Willmitzer L, Altmann T (1996) Genetic evidence for an essential role of brassinosteroids in plant development. Plant J 9(5):701鈥?13 CrossRef
37. Kende H, Lang A (1964) Gibberellins and light inhibition of stem growth in peas. Plant Physiol 39(3):435 CrossRef
38. Kim T-W, Wang Z-Y (2010) Brassinosteroid signal transduction from receptor kinases to transcription factors. Annu Rev Plant Biol 61:681鈥?04 CrossRef
39. Koorneef M, Elgersma A, Hanhart C, Loenen-Martinet E-v, Rijn L v, Zeevaart J (1985) A gibberellin insensitive mutant of / Arabidopsis thaliana. Physiol Plant 65(1):33鈥?9 CrossRef
40. Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5(2):150鈥?63 CrossRef
41. Lang A (1957) The effect of gibberellin upon flower formation. Proc Natl Acad Sci USA 43(8):709 CrossRef
42. Lemieux C, Firoozabady E, Robinson K, Jong JD (1990) Agrobacterium-mediated transformation of chrysanthemum. In: Integration of in vitro techniques in ornamental plant breeding. Proceedings, symposium, 10鈥?4 November 1990. EUCARPIA, pp 150鈥?55
43. Li J, Chory J (1997) A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell 90(5):929鈥?38 CrossRef
44. Magome H, Yamaguchi S, Hanada A, Kamiya Y, Oda K (2004) dwarf and delayed-flowering 1, a novel Arabidopsis mutant deficient in gibberellin biosynthesis because of overexpression of a putative AP2 transcription factor. Plant J 37(5):720鈥?29 CrossRef
45. Malaure R, Barclay G, Power J, Davey M (1991) The production of novel plants from florets of / Chrysanthemum morifolium using tissue culture. 1. Shoot regeneration from ray florets and somaclonal variation exhibited by the regenerated plants. J Plant Physiol 139(1):8鈥?3 CrossRef
46. Mandava NB (1988) Plant growth-promoting brassinosteroids. Annu Rev Plant Physiol Plant Mol Biol 39(1):23鈥?2 CrossRef
47. Mandel MA, Yanofsky MF (1995) A gene triggering flower formation in Arabidopsis. Nature 377(6549):522鈥?24 CrossRef
48. Moon J, Suh SS, Lee H, Choi KR, Hong CB, Paek NC, Kim SG, Lee I (2003) The SOC1 MADS-box gene integrates vernalization and gibberellin signals for flowering in Arabidopsis. Plant J 35(5):613鈥?23 CrossRef
49. Noguchi T, Fujioka S, Choe S, Takatsuto S, Yoshida S, Yuan H, Feldmann KA, Tax FE (1999) Brassinosteroid-insensitive dwarf mutants of Arabidopsis accumulate brassinosteroids. Plant Physiol 121(3):743鈥?52 CrossRef
50. Oda A, Narumi T, Li T, Kando T, Higuchi Y, Sumitomo K, Fukai S, Hisamatsu T (2012) CsFTL3, a chrysanthemum FLOWERING LOCUS T-like gene, is a key regulator of photoperiodic flowering in chrysanthemums. J Exp Bot 63(3):1461鈥?477 CrossRef
51. Ohmiya A, Kishimoto S, Aida R, Yoshioka S, Sumitomo K (2006) Carotenoid cleavage dioxygenase (CmCCD4a) contributes to white color formation in chrysanthemum petals. Plant Physiol 142(3):1193鈥?201 CrossRef
52. Olszewski N, T-p Sun, Gubler F (2002) Gibberellin signaling biosynthesis, catabolism, and response pathways. Plant Cell Online 14(suppl 1):S61鈥揝80
53. Parcy F, Nilsson O, Busch MA, Lee I, Weigel D (1998) A genetic framework for floral patterning. Nature 395(6702):561鈥?66 CrossRef
54. Pavingerov谩 D, Dost谩l J, B铆skov谩 R, Benetka V (1994) Somatic embryogenesis and / Agrobacterium-mediated transformation of chrysanthemum. Plant Sci 97(1):95鈥?01 CrossRef
55. Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, Flintham JE, Beales J, Fish LJ, Worland AJ, Pelica F (1999a) 鈥楪reen revolution鈥?genes encode mutant gibberellin response modulators. Nature 400(6741):256鈥?60 CrossRef
56. Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, Flintham JE, Beales J, Fish LJ, Worland AJ, Pelica F (1999b) 鈥楪reen revolution鈥?genes encode mutant gibberellin response modulators. Nature 400(6741):256鈥?61 CrossRef
57. Pharis RP, King RW (1985) Gibberellins and reproductive development in seed plants. Annu Rev Plant Physiol 36(1):517鈥?68 CrossRef
58. Qiao F, Zhao K-J (2011) The influence of RNAi targeting of OsGA20ox2 gene on plant height in rice. Plant Mol Biol Report 29(4):952鈥?60 CrossRef
59. Ross JJ, O鈥橬eill DP, Wolbang CM, Symons GM, Reid JB (2001) Auxin-gibberellin interactions and their role in plant growth. J Plant Growth Regul 20(4):336鈥?53 CrossRef
60. Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, Swapan D, Ishiyama K, Saito T, Kobayashi M, Khush G (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416(6882):701鈥?02 CrossRef
61. Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, Swapan D, Ishiyama K, Saito T, Kobayashi M, Khush G (2002b) A mutant gibberellin-synthesis gene in rice. Nature 416(6882):701 CrossRef
62. Schlagnhaufer CD, Arteca RN (1985) Brassinosteroid-induced epinasty in tomato plants. Plant Physiol 78(2):300鈥?03 CrossRef
63. Scott JW (1989) Micro-Tom鈥攁 miniature dwarf tomato. Florida Agric Exp Stn Circ 370:1鈥?
64. Seiichim F, de Jongzl J, Rademakerz W (1995) Efficient genetic transformation of chrysanthemum ( / Dendranthema grandiflorum (Ramat.) Kitamura) using stem segments. Breed Sci 45:179鈥?84
65. Shchennikova A, Shulga O, Angenent G, Skryabin K (2003) Genetic regulation of inflorescence development in Chrysanthemum. Dokl Biol Sci 391:368鈥?70 CrossRef
66. Shchennikova AV, Shulga OA, Immink R, Skryabin KG, Angenent GC (2004) Identification and characterization of four chrysanthemum MADS-box genes, belonging to the APETALA1/FRUITFULL and SEPALLATA3 subfamilies. Plant Physiol 134(4):1632鈥?641 CrossRef
67. Sherman JM, Moyer JW, Daub ME (1998) A regeneration and Agrobacterium-mediated transformation system for genetically diverse / Chrysanthemum cultivars. J Am Soc Hortic Sci 123(2):189鈥?94
68. Shimada Y, Fujioka S, Miyauchi N, Kushiro M, Takatsuto S, Nomura T, Yokota T, Kamiya Y, Bishop GJ, Yoshida S (2001) Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis. Plant Physiol 126(2):770鈥?79 CrossRef
69. Smith J, Kamp-Glass M (1990) Gibberelin promotes flower stem elongation on the chrysanthemums. HortScience 25(9):1118
70. Souter M, Topping J, Pullen M, Friml J, Palme K, Hackett R, Grierson D, Lindsey K (2002) Hydra mutants of Arabidopsis are defective in sterol profiles and auxin and ethylene signaling. Plant Cell Online 14(5):1017鈥?031 CrossRef
71. Spielmeyer W, Ellis MH, Chandler PM (2002) Semidwarf (sd-1), 鈥済reen revolution鈥?rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci USA 99(13):9043鈥?048 CrossRef
72. Sui J-M, Guo B-T, Wang J-S, Qiao L-X, Zhou Y, Zhang H-G, Gu M-H, Liang G-H (2012) A new GA-insensitive semidwarf mutant of rice ( / Oryza sativa L.) with a missense mutation in the SDG gene. Plant Mol Biol Report 30(1):187鈥?94 CrossRef
73. Szekeres M, N茅meth K, Koncz-K谩lm谩n Z, Mathur J, Kauschmann A, Altmann T, R茅dei GP, Nagy F, Schell J, Koncz C (1996) Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell 85(2):171鈥?82 CrossRef
74. Takatsu Y, Tomotsune H, Kasumi M, Sakuma F (1998) Differences in adventitious shoot regeneration capacity among Japanese chrysanthemum ( / Dendranthema grandiflorum (Ramat.) Kitamura) cultivars and the improved protocol for Agrobacterium-mediated genetic transformation. J Jpn Soc Hortic Sci 67(6):958鈥?64 CrossRef
75. Teixeira da Silva JA (2003) / Chrysanthemum: advances in tissue culture, cryopreservation, postharvest technology, genetics and transgenic biotechnology. Biotechnol Adv 21(8):715鈥?66 CrossRef
76. Topping JF, May VJ, Muskett PR, Lindsey K (1997) Mutations in the HYDRA1 gene of Arabidopsis perturb cell shape and disrupt embryonic and seedling morphogenesis. Development 124(21):4415鈥?424
77. Tyler L, Thomas SG, Hu J, Dill A, Alonso JM, Ecker JR, Sun T-p (2004) DELLA proteins and gibberellin-regulated seed germination and floral development in Arabidopsis. Plant Physiol 135(2):1008鈥?019 CrossRef
78. van Wordragen MF, de Jong J, Huitema HB, Dons HJ (1991) Genetic transformation of chrysanthemum using wild type Agrobacterium strains; strain and cultivar specificity. Plant Cell Rep 9(9):505鈥?08 CrossRef
79. Wilson RN, Heckman JW, Somerville CR (1992) Gibberellin is required for flowering in / Arabidopsis thaliana under short days. Plant Physiol 100(1):403鈥?08 CrossRef
80. Wong LM, Abel S, Shen N, Foata M, Mall Y, Theologis A (1996) Differential activation of the primary auxin response genes, PS-IAA4/5 and PS-IAA6, during early plant development. Plant J 9(5):587鈥?99 CrossRef
81. Xu W, Purugganan MM, Polisensky DH, Antosiewicz DM, Fry SC, Braam J (1995) Arabidopsis TCH4, regulated by hormones and the environment, encodes a xyloglucan endotransglycosylase. The Plant Cell Online 7(10):1555鈥?567 CrossRef
82. Yin C, Gan L, Ng D, Zhou X, Xia K (2007) Decreased panicle-derived indole-3-acetic acid reduces gibberellin A1 level in the uppermost internode, causing panicle enclosure in male sterile rice Zhenshan 97A. J Exp Bot 58(10):2441鈥?449 CrossRef
83. Zhang Y, Lian L, Liu Q, Xiao N, Fang R, Liu Q, Chen X (2013) Altered expression of CmNRRa changes flowering time of / Chrysanthemum morifolium. Plant Biotechnol J 11(3):373鈥?79 CrossRef - 作者单位：Qiaoli Xie (1)
Guoping Chen (1)
Qin Liu (1)
Zhiguo Zhu (1)
Zongli Hu (1)
1. Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Campus A, Chongqing University, 174 Shapingba Main Street, Chongqing, 400044, People鈥檚 Republic of China - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Plant Sciences - 出版者：Springer Netherlands
- ISSN：1572-9788
文摘
Chrysanthemums (Dendranthema morifolium) are one of the most economically important perennial flowering plants, with floricultural (cut flowers), ornamental crop (pot and garden flowers) and, for some cultivars, medicinal uses. Plant architecture is an important agronomic trait for plants with a high ornamental and economic value. In this study, two miniature-related genes, DmCPD and DmGA20ox, were cloned and their tissue-specific expression patterns were analyzed. The results showed that the two genes were both highly expressed in stems, mature leaves, and flowers, and that DmCPD was also highly expressed in pedicels. To generate miniature plants, an RNAi expression vector targeting both DmCPD and DmGA20ox was constructed and transformed into chrysanthemum plants. Smaller plant size and slower growth and development of flowers were observed in dual-silenced chrysanthemums. Brassinosteroid and gibberellin contents in leaves and flower buds of transgenic plants were significantly decreased. Furthermore, the expressions of brassinolide-, gibberellin-, and flowering-related genes were down-regulated by varying degrees in dual-silenced plants. These results suggest that DmCPD and DmGA20ox play important roles in plant architecture, and brassinolide and gibberellin are important hormones in controlling plant architecture. This miniaturization strategy provides an efficient approach for generating new varieties of ornamental plants and crops.