Microbial Diversity of Chromium-Contaminated Soils and Characterization of Six Chromium-Removing Bacteria

详细信息    查看全文

• 作者：Zhiguo He ; Yuting Hu ; Zhen Yin ; Yuehua Hu ; Hui Zhong
• 刊名：Environmental Management
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：57
• 期：6
• 页码：1319-1328
• 全文大小：801 KB
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environmental Management
  Ecology
  Nature Conservation
  Atmospheric Protection, Air Quality Control and Air Pollution
  Forestry Management
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  
• 出版者：Springer New York
• ISSN：1432-1009
• 卷排序：57

文摘

Three soil samples obtained from different sites adjacent to a chromium slag heap in a steel alloy factory were taken to examine the effect of chromium contamination on soil bacterial diversity as determined by construction of 16S rDNA clone libraries and sequencing of selected clones based on restriction fragment length polymorphism (RFLP) analysis. Results revealed that Betaproteobacteria, Gammaproteobacteria, Firmicutes, and Alphaproteobacteria occurred in all three soil samples, although the three samples differed in their total diversity. Sample 1 had the highest microbial diversity covering 12 different classes, while Sample 3 had the lowest microbial diversity. Strains of six different species were successfully isolated, one of which was identified as Zobellella denitrificans. To our knowledge, this is the first report of a strain belonging to the genus Zobellella able to resist and reduce chromium. Among all isolates studied, Bacillus odysseyi YH2 exhibited the highest Cr(VI)-reducing capability, with a total removal of 23.5 % of an initial Cr(VI) concentration of 350 mg L−1.KeywordsBacterial diversity16S rRNA geneChromate-reducing bacteriaChromate reduction