Rapid estimation of lipid content in an Antarctic ice alga (Chlamydomonas sp.) using the lipophilic fluorescent dye BODIPY505/515
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- 作者：Shanli Mou (1)
Dong Xu (2)
Naihao Ye (2)
Xiaowen Zhang (2)
Chengwei Liang (3)
Qiang Liang (1)
Zhou Zheng (1)
Zhimeng Zhuang (2)
Jinlai Miao (1) - 关键词：Chlamydomonas sp. ICE ; L ; Lipid content ; BODIPY505/515 ; Rapid estimation
- 刊名：Journal of Applied Phycology
- 出版年：2012
- 出版时间：October 2012
- 年：2012
- 卷：24
- 期：5
- 页码：1169-1176
- 全文大小：336KB
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Dong Xu (2)
Naihao Ye (2)
Xiaowen Zhang (2)
Chengwei Liang (3)
Qiang Liang (1)
Zhou Zheng (1)
Zhimeng Zhuang (2)
Jinlai Miao (1)
1. Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, Qingdao, 266061, China
2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China
3. Qingdao University of Science and Technology, Qingdao, 266042, China
文摘
Chlamydomonas sp. ICE-L, isolated from Antarctic coastal marine environments, was selected as a high lipid producer, which may be useful for biodiesel production. The lipophilic fluorescent dye BODIPY505/515 was used to determine the algal lipid content. Lipid bodies stained with BODIPY505/515 have a characteristic green fluorescence, and their volumes were determined using the sphere volume formula. In this study, lipid accumulation by Chlamydomonas ICE-L was analyzed under different cultivation conditions (nitrogen deficiency and UV-B radiation). The results demonstrated that nitrogen deficiency and UV-B radiation could significantly promote the accumulation of lipid content per cell. The highest yields of total lipid content (reaching 84?μL?L?) were obtained in full Provasoli medium after 12?days of cultivation, but not in the nitrogen-deficient medium. The inoculum used in this experiment was obtained from the late-exponential growth phase. The main reason was that the cell numbers in nitrogen-deficient medium had not increased and total lipid contents were offset by the lower growth rate. Considering the high lipid content in Chlamydomonas sp. ICE-L, this alga might be a promising alternative species for production of microalgal oil for the production of renewable biodiesel in the future.