Molecular characterization of a Hog1-type MAPK, MlpHog1, from Melampsora larici-populina

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• 作者：Dan Yu ; Ruixi Li ; Zhongdong Yu ; Zhimin Cao
• 刊名：Phytoparasitica
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：44
• 期：2
• 页码：251-259
• 全文大小：3,430 KB
• 刊物主题：Plant Pathology; Plant Sciences; Ecology; Agriculture; Life Sciences, general;
• 出版者：Springer Netherlands
• ISSN：1876-7184
• 卷排序：44

文摘

The obligate biotrophic fungus Melampsora larici-populina (Mlp) causes devastating poplar foliar rust disease. In pathogens, the Hog1-type mitogen-activated protein kinase (MAPK) gene is involved in both pathogenesis and the response to various stresses, including osmotic stress. However, there are few reports on its role in M. larici-populina. In the present study, we identified and characterized the Hog1-type MAPK gene, MlpHog1, in M. larici-populina. The MlpHog1 protein consists of 356 amino acids and contains a conserved TGY motif. The function of MlpHog1 was probed by mutant complementation studies. Expression of MlpHog1 could partially complement the osmoregulation, sexual reproduction and pathogenicity defects of the Fusarium graminearum Hog1 mutant and largely rescued the response of the mutant to cytoplasm membrane and oxidative stresses. These observations suggest that MlpHog1 may play a role in the infectious growth of M. larici-populina and the response of the fungus to various environmental stresses.KeywordsPoplar foliar rustMelampsora larici-populinaHog1-type mitogen-activated protein kinaseFunction