Proteomic analysis of differentially expressed proteins in hepatitis B virus-related hepatocellular carcinoma tissues

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• 作者：Ning Li (1)
  Yunzhu Long (2)
  Xuegong Fan (3)
  Hongbo Liu (3)
  Cui Li (4)
  Lizhang Chen (5)
  Zhiming Wang (6)
  
• 刊名：Journal of Experimental & Clinical Cancer Research
• 出版年：2009
• 出版时间：December 2009
• 年：2009
• 卷：28
• 期：1
• 全文大小：1649KB
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• 作者单位：Ning Li (1)
  Yunzhu Long (2)
  Xuegong Fan (3)
  Hongbo Liu (3)
  Cui Li (4)
  Lizhang Chen (5)
  Zhiming Wang (6)
  
  1. Department of Blood transfusion, Xiangya Hospital, Central South University, 410008, Changsha, PR China
  2. Department of Infectious Diseases, The First Hospital of Zhuzhou, 412000, Zhuzhou, PR China
  3. Department of Infectious Diseases, Xiangya Hospital, Central South University, 410008, Changsha, PR China
  4. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, 聽, 410008, Changsha, PR China
  5. School of Public Health, Central South University, 410078, Changsha, PR China
  6. Department of Surgery, Xiangya Hospital, Central South University, 410008, Changsha, PR China
  
• ISSN：1756-9966

文摘

Background Hepatocellular carcinoma (HCC), a major cause of cancer death in China, is preceded by chronic hepatitis and liver cirrhosis (LC). Although hepatitis B virus (HBV) has been regarded as a clear etiology of human hepatocarcinogenesis, the mechanism is still needs to be further clarified. In this study, we used a proteomic approach to identify the differential expression protein profiles between HCC and the adjacent non-tumorous liver tissues. Methods Eighteen cases of HBV-related HCC including 12 cases of LC-developed HCC and 6 cases of chronic hepatitis B (CHB)-developed HCC were analyzed by two-dimensional electrophoresis (2-DE) combined with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), and the results were compared to those of paired adjacent non-tumorous liver tissues. Results A total of 17 differentially expressed proteins with diverse biological functions were identified. Among these, 10 proteins were up-regulated, whereas the other 7 proteins were down-regulated in cancerous tissues. Two proteins, c-Jun N-terminal kinase 2 and ADP/ATP carrier protein were found to be up-regulated only in CHB-developed HCC tissues. Insulin-like growth factor binding protein 2 and Rho-GTPase-activating protein 4 were down-regulated in LC-developed and CHB-developed HCC tissues, respectively. Although 11 out of these 17 proteins have been already described by previous studies, or are already known to be involved in hepatocarcinogenesis, this study revealed 6 new proteins differentially expressed in HBV-related HCC. Conclusion These findings elucidate that there are common features between CHB-developed HCC and LC-developed HCC. The identified proteins are valuable for studying the hepatocarcinogenesis, and may be potential diagnostic markers or therapeutic targets for HBV-related HCC.