A new strategy for full-length Ebola virus glycoprotein expression in E.coli

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• 作者：Junjie Zai ; Yinhua Yi ; Han Xia ; Bo Zhang ; Zhiming Yuan
• 关键词：Ebola virus (EBOV) ; glycoprotein (GP) ; E.coli ; prokaryotic expression
• 刊名：Virologica Sinica
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：31
• 期：6
• 页码：500-508
• 全文大小：
• 刊物主题：Virology; Medical Microbiology; Oncology; Biochemistry, general; Microbiology; Microbial Genetics and Genomics;
• 出版者：Springer Singapore
• ISSN：1995-820X
• 卷排序：31

文摘

Ebola virus (EBOV) causes severe hemorrhagic fever in humans and non-human primates with high rates of fatality. Glycoprotein (GP) is the only envelope protein of EBOV, which may play a critical role in virus attachment and entry as well as stimulating host protective immune responses. However, the lack of expression of full-length GP in Escherichia coli hinders the further study of its function in viral pathogenesis. In this study, the vp40 gene was fused to the full-length gp gene and cloned into a prokaryotic expression vector. We showed that the VP40-GP and GP-VP40 fusion proteins could be expressed in E.coli at 16 °C. In addition, it was shown that the position of vp40 in the fusion proteins affected the yields of the fusion proteins, with a higher level of production of the fusion protein when vp40 was upstream of gp compared to when it was downstream. The results provide a strategy for the expression of a large quantity of EBOV full-length GP, which is of importance for further analyzing the relationship between the structure and function of GP and developing an antibody for the treatment of EBOV infection.KeywordsEbola virus (EBOV)glycoprotein (GP)E.coliprokaryotic expressionORCID: 0000-0002-3234-9616