Purification and partial characterization of intact and truncated chitinase from Bacillus thuringiensis HZP7 expressed in Escherichia coli

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• 作者：Li Sha ; Ensi Shao ; Xiong Guan ; Zhipeng Huang
• 关键词：Bacillus thuringiensis ; Chitin ; binding domain ; Enzymatic characterization ; Fibronectin type III domain ; Truncated chitinase
• 刊名：Biotechnology Letters
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：38
• 期：2
• 页码：279-284
• 全文大小：1,125 KB
• 参考文献：Barboza-Corona JE, Contreras JC, Velazquez-Robledo R et al (1999) Selection of chitinolytic strains of Bacillus thuringiensis. Biotechnol Lett 21:1125–1129CrossRef
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• 作者单位：Li Sha (1) (2)
  Ensi Shao (1) (3)
  Xiong Guan (1)
  Zhipeng Huang (1) (2)
  
  1. Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, People’s Republic of China
  2. School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, People’s Republic of China
  3. National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Objective To ascertain the effect of chitin-binding domain (ChBD) and fibronectin type III domain (FN3) on the characterization of the intact chitinase from Bacillus thuringiensis.