Isolation and Characterization of a Calcium-Dependent Protein Kinase Gene, FvCDPK1, Responsive to Abiotic Stress in Woodland Strawberry (Fragaria vesca)
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- 作者：Jiayue Feng (1)
Jing Li (2)
Hong Liu (2)
Qinghua Gao (2)
Ke Duan (2)
Zhirong Zou (1) - 关键词：Strawberry ; Calcium ; dependent protein kinase (CDPK) ; Abiotic stress ; Gene expression
- 刊名：Plant Molecular Biology Reporter
- 出版年：2013
- 出版时间：April 2013
- 年：2013
- 卷：31
- 期：2
- 页码：443-456
- 全文大小：1486KB
- 参考文献：1. Abbasi F, Onodera H, Toki S, Tanaka H, Komatsu S (2004) / OsCDPK13, a calcium-dependent protein kinase gene from rice, is induced by cold and gibberellin in rice leaf sheath. Plant Mol Biol 55:541-52 CrossRef
2. Arimura G, Maffei ME (2010) Calcium and secondary CPK signaling in plants in response to herbivore attack. Biochem Biophys Res Commun 400:455-60 CrossRef
3. Arnold K, Bordoli L, Kopp J, Schwede T (2006) The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22:195-01 CrossRef
4. Asano T, Tanaka N, Yang G, Hayashi N, Komatsu S (2005) Genome-wide identification of the rice calcium-dependent protein kinase and its closely related kinase gene families: comprehensive analysis of the CDPKs gene family in rice. Plant Cell Physiol 46:356-66 CrossRef
5. Boudsocq M, Willmann MR, McCormack M, Lee H, Shan L, He P, Bush J, Cheng SH, Sheen J (2010) Differential innate immune signalling via Ca2+ sensor protein kinases. Nature 464:418-22 CrossRef
6. Chang S, Puryear J, Cairney J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11:113-16 CrossRef
7. Cheng SH, Willmann MR, Chen HC, Sheen J (2002) Calcium signaling through protein kinases. The / Arabidopsis calcium-dependent protein kinase gene family. Plant Physiol 129:469-85 CrossRef
8. Choi HI, Park HJ, Park JH, Kim S, Im MY, Seo HH, Kim YW, Hwang I, Kim SY (2005) / Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity. Plant Physiol 139:1750-761 CrossRef
9. Coca M, San Segundo B (2010) AtCPK1 calcium-dependent protein kinase mediates pathogen resistance in / Arabidopsis. Plant J 63:526-40 CrossRef
10. Combet C, Blanchet C, Geourjon C, Deleage G (2000) NPS@: network protein sequence analysis. Trends Biochem Sci 25:147-50 CrossRef
11. Debreczeni JE, Bullock A, Keates T, Niesen FH, Salah E, Shrestha L, Smee C, Sobott F, Pike ACW, Bunkoczi G, Von Delft F, Turnbull A, Weigelt J, Arrowsmith CH, Edwards A, Sundstrom M, Knapp S (2012) Crystal structure of human calmodulin-dependent protein kinase IG. doi:10.2210/pdb2jam/pdb , http://www.rcsb.org/pdb/explore/explore.do?structureId=2JAM
12. DeFalco TA, Bender KW, Snedden WA (2010) Breaking the code: Ca2+ sensors in plant signalling. Biochem J 425:27-0 CrossRef
13. Dodd AN, Kudla J, Sanders D (2010) The language of calcium signaling. Annu Rev Plant Biol 61:593-20 CrossRef
14. Franz S, Ehlert B, Liese A, Kurth J, Cazale AC, Romeis T (2011) Calcium-dependent protein kinase CPK21 functions in abiotic stress response in / Arabidopsis thaliana. Mol Plant 4:83-6 CrossRef
15. Frylinck L, Dubery IA (1998) Protein kinase activities in ripening mango, / Mangifera indica L., fruit tissue. III. Purification and characterisation of a calcium-regulated protein kinase. Biochim Biophys Acta 1387:342-54 CrossRef
16. Hanks SK, Quinn AM, Hunter T (1988) The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241:42-2 CrossRef
17. Harmon AC, Gribskov M, Harper JF (2000) CDPKs-a kinase for every Ca2+ signal? Trends Plant Sci 5:154-59 CrossRef
18. Harper JF, Harmon A (2005) Plants, symbiosis and parasites: a calcium signalling connection. Nat Rev Mol Cell Biol 6:555-66 CrossRef
19. Hrabak EM, Chan CW, Gribskov M, Harper JF, Choi JH, Halford N, Kudla J, Luan S, Nimmo HG, Sussman MR, Thomas M, Walker-Simmons K, Zhu JK, Harmon AC (2003) The / Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol 132:666-80 CrossRef
20. Huang QS, Wang HY, Gao P, Wang GY, Xia GX (2008) Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development. Plant Cell Rep 27:1869-875 CrossRef
21. Husaini AM, Rafiqi AM (2012) Role of osmotin in strawberry improvement. Plant Mol Biol Rep. doi:10.1007/s11105-011-0394-2
22. Ishida S, Yuasa T, Nakata M, Takahashi Y (2008) A tobacco calcium-dependent protein kinase, CDPK1, regulates the transcription factor REPRESSION OF SHOOT GROWTH in response to gibberellins. Plant Cell 20:3273-288 CrossRef
23. Kanchiswamy CN, Takahashi H, Quadro S, Maffei ME, Bossi S, Bertea C, Zebelo SA, Muroi A, Ishihama N, Yoshioka H, Boland W, Takabayashi J, Endo Y, Sawasaki T, Arimura G (2010) Regulation of / Arabidopsis defense responses against / Spodoptera littoralis by CPK-mediated calcium signaling. BMC Plant Biol 10:97 CrossRef
24. Kim KN, Cheong YH, Grant JJ, Pandey GK, Luan S (2003) CIPK3, a calcium sensor-associated protein kinase that regulates abscisic acid and cold signal transduction in / Arabidopsis. Plant Cell 15:411-23 CrossRef
25. Kim MC, Chung WS, Yun DJ, Cho MJ (2009) Calcium and calmodulin-mediated regulation of gene expression in plants. Mol Plant 2:13-1 CrossRef
26. Klimecka M, Muszynska G (2007) Structure and functions of plant calcium-dependent protein kinases. Acta Biochim Pol 54:219-33
27. Kosuta S, Hazledine S, Sun J, Miwa H, Morris RJ, Downie JA, Oldroyd GE (2008) Differential and chaotic calcium signatures in the symbiosis signaling pathway of legumes. Proc Natl Acad Sci U S A 105:9823-828 CrossRef
28. Lecourieux D, Ranjeva R, Pugin A (2006) Calcium in plant defence-signalling pathways. New Phytol 171:249-69 CrossRef
29. Lee SS, Cho HS, Yoon GM, Ahn JW, Kim HH, Pai HS (2003) Interaction of NtCDPK1 calcium-dependent protein kinase with NtRpn3 regulatory subunit of the 26S proteasome in / Nicotiana tabacum. Plant J 33:825-40 CrossRef
30. Li AL, Zhu YF, Tan XM, Wang X, Wei B, Guo HZ, Zhang ZL, Chen XB, Zhao GY, Kong XY, Jia JZ, Mao L (2008) Evolutionary and functional study of the CDPK gene family in wheat ( / Triticum aestivum L.). Plant Mol Biol 66:429-43 CrossRef
31. Llop-Tous I, Dominguez-Puigjaner E, Vendrell M (2002) Characterization of a strawberry cDNA clone homologous to calcium-dependent protein kinases that is expressed during fruit ripening and affected by low temperature. J Exp Bot 53:2283-285 CrossRef
32. Lu B, Ding R, Zhang L, Yu X, Huang B, Chen W (2006) Molecular cloning and characterization of a novel calcium-dependent protein kinase gene / IiCPK2 responsive to polyploidy from tetraploid / Isatis indigotica. J Biochem Mol Biol 39:607-17 CrossRef
33. Ludwig AA, Romeis T, Jones JD (2004) CDPK-mediated signalling pathways: specificity and cross-talk. J Exp Bot 55:181-88 CrossRef
34. Ma SY, Wu WH (2007) AtCPK23 functions in / Arabidopsis responses to drought and salt stresses. Plant Mol Biol 65:511-18 CrossRef
35. Mazars C, Bourque S, Mithofer A, Pugin A, Ranjeva R (2009) Calcium homeostasis in plant cell nuclei. New Phytol 181:261-74 CrossRef
36. McAinsh MR, Pittman JK (2009) Shaping the calcium signature. New Phytol 181:275-94 CrossRef
37. Mehlmer N, Wurzinger B, Stael S, Hofmann-Rodrigues D, Csaszar E, Pfister B, Bayer R, Teige M (2010) The Ca2+-dependent protein kinase CPK3 is required for MAPK-independent salt-stress acclimation in / Arabidopsis. Plant J 63:484-98 CrossRef
38. Mori IC, Murata Y, Yang Y, Munemasa S, Wang YF, Andreoli S, Tiriac H, Alonso JM, Harper JF, Ecker JR, Kwak JM, Schroeder JI (2006) CDPKs CPK6 and CPK3 function in ABA regulation of guard cell S-type anion-and Ca2+-permeable channels and stomatal closure. PLoS Biol 4:e327 CrossRef
39. Munemasa S, Hossain MA, Nakamura Y, Mori IC, Murata Y (2011) The / Arabidopsis calcium-dependent protein kinase, CPK6, functions as a positive regulator of methyl jasmonate signaling in guard cells. Plant Physiol 155:553-61 CrossRef
40. Myers C, Romanowsky SM, Barron YD, Garg S, Azuse CL, Curran A, Davis RM, Hatton J, Harmon AC, Harper JF (2009) Calcium-dependent protein kinases regulate polarized tip growth in pollen tubes. Plant J 59:528-39 CrossRef
41. Raices M, Chico JM, Tellez-Inon MT, Ulloa RM (2001) Molecular characterization of StCDPK1, a calcium-dependent protein kinase from / Solanum tuberosum that is induced at the onset of tuber development. Plant Mol Biol 46:591-01 CrossRef
42. Saijo Y, Hata S, Kyozuka J, Shimamoto K, Izui K (2000) Over-expression of a single Ca2+-dependent protein kinase confers both cold and salt/drought tolerance on rice plants. Plant J 23:319-27 CrossRef
43. Shapiro MB, Senapathy P (1987) RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression. Nucleic Acids Res 15:7155-174 CrossRef
44. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596-599 CrossRef
45. Urao T, Katagiri T, Mizoguchi T, Yamaguchi-Shinozaki K, Hayashida N, Shinozaki K (1994) Two genes that encode Ca2+-dependent protein kinases are induced by drought and high-salt stresses in / Arabidopsis thaliana. Mol Gen Genet 244:331-40 CrossRef
46. Wan B, Lin Y, Mou T (2007) Expression of rice Ca2+-dependent protein kinases (CDPKs) genes under different environmental stresses. FEBS Lett 581:1179-189 CrossRef
47. Wang M, Wang J, Liu H, Li J, Duan K, Ye ZW, Gao QH (2011) Gene cloning and expression analysis of calcium-dependent protein kinase in strawberry. Acta Bot Boreal-Occident Sin 31:0875-880 (in Chinese with English abstract)
48. Wurzinger B, Mair A, Pfister B, Teige M (2011) Cross-talk of calcium-dependent protein kinase and MAP kinase signaling. Plant Signal Behav 6:8-2 CrossRef
49. Xu J, Tian YS, Peng RH, Xiong AS, Zhu B, Jin XF, Gao F, Fu XY, Hou XL, Yao QH (2010) AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in / Arabidopsis. Planta 231:1251-260 CrossRef
50. Yang T, Poovaiah BW (2003) Calcium/calmodulin-mediated signal network in plants. Trends Plant Sci 8:505-12 CrossRef
51. Yang C, Li AL, Zhao YL, Zhang ZL, Zhu YF, Tan XM, Geng SF, Guo HZ, Zhang XY, Kang ZS, Mao L (2011) Overexpression of a wheat / CCaMK gene deduces ABA sensitivity of / Arabidopsis thaliana during seed germination and seedling Growth. Plant Mol Biol Rep 29:681-92 CrossRef
52. Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283-33 CrossRef
53. Zhao R, Sun HL, Mei C, Wang XJ, Yan L, Liu R, Zhang XF, Wang XF, Zhang DP (2011) The / Arabidopsis Ca2+-dependent protein kinase CPK12 negatively regulates abscisic acid signaling in seed germination and post-germination growth. New Phytol 192:61-3 CrossRef
54. Zhou G, Yang LT, Li YR, Zou CL, Huang LP, Qiu LH, Huang X, Srivastava MK (2012) Proteomic analysis of osmotic stress responsive proteins in sugarcane leaves. Plant Mol Biol Rep 30:349-59 CrossRef
55. Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247-73 CrossRef
56. Zhu SY, Yu XC, Wang XJ, Zhao R, Li Y, Fan RC, Shang Y, Du SY, Wang XF, Wu FQ, Xu YH, Zhang XY, Zhang DP (2007) Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in / Arabidopsis. Plant Cell 19:3019-036 CrossRef
57. Zou JJ, Wei FJ, Wang C, Wu JJ, Ratnasekera D, Liu WX, Wu WH (2010) / Arabidopsis calcium-dependent protein kinase CPK10 functions in abscisic acid-and Ca2+-mediated stomatal regulation in response to drought stress. Plant Physiol 154:1232-243 CrossRef - 作者单位：Jiayue Feng (1)
Jing Li (2)
Hong Liu (2)
Qinghua Gao (2)
Ke Duan (2)
Zhirong Zou (1)
1. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China
2. Shanghai Key Laboratory of Protected Horticultural Technology, Forestry and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai, 201403, People’s Republic of China - ISSN：1572-9818
文摘
Plant calcium-dependent protein kinases (CDPKs) play vital roles in calcium signal transduction during various developmental processes and during responses to biotic and abiotic stresses. Here, we isolated and characterized a CDPK gene designated FvCDPK1 from a wild diploid strawberry accession Heilongjiang-3 (Fragaria vesca L.). The FvCDPK1 gene contains 12 exons and 11 introns, and the sequences of most exons are highly conserved in higher plants. The full-length cDNA of FvCDPK1 contains 1,825 nucleotides with an open reading frame of 1,653?bp encoding a polypeptide of 550 amino acids. The deduced FvCDPK1 protein contains the basic features of typical plant CDPKs: a catalytic kinase domain and a regulatory calmodulin-like domain containing four EF-hand calcium-binding motifs. Phylogenetic analysis confirmed that FvCDPK1 belongs to the plant CDPK family. When transiently expressed in onion epidermal cells, the FvCDPK1-GFP fusion protein was found to be localized in the nucleus. Expression analysis indicated that FvCDPK1 was expressed in fruits at different developmental and ripening stages, as well as in several tissues such as roots, runners, flowers, leaves, and meristems. Moreover, expression levels of FvCDPK1 were higher in meristems than in other vegetative tissues. Under abiotic stress conditions, however, FvCDPK1 was found to be upregulated upon abscisic acid, NaCl, cold-, or high-temperature treatments. Taken together, our data suggest that FvCDPK1 might play a role in various responses to abiotic stresses in strawberry.